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Evaluation of the RAPIDEC® CARBA NP, the Rapid CARB Screen® and the Carba NP test for biochemical detection of carbapenemase-producing Enterobacteriaceae

  • Dortet, Laurent Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France - Research Unit ‘Structure, dynamic, function and expression of broad spectrum β-lactamases’, Faculty of Medicine, University of Paris-Sud, Le Kremlin-Bicêtre, France - Department of Bacteriology-Parasitology-Hygiene, Hôpital de Bicêtre, Assistance Publique - Hôpitaux de Paris, Le Kremlin-Bicêtre, France
  • Agathine, Aurélie Research Unit ‘Structure, dynamic, function and expression of broad spectrum β-lactamases’, Faculty of Medicine, University of Paris-Sud, Le Kremlin-Bicêtre, France - Department of Bacteriology-Parasitology-Hygiene, Hôpital de Bicêtre, Assistance Publique - Hôpitaux de Paris, Le Kremlin-Bicêtre, France
  • Naas, Thierry Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France - Research Unit ‘Structure, dynamic, function and expression of broad spectrum β-lactamases’, Faculty of Medicine, University of Paris-Sud, Le Kremlin-Bicêtre, France - Department of Bacteriology-Parasitology-Hygiene, Hôpital de Bicêtre, Assistance Publique - Hôpitaux de Paris, Le Kremlin-Bicêtre, France
  • Cuzon, Gaëlle Associated French National Reference Center for Antibiotic Resistance, Le Kremlin-Bicêtre, France - Research Unit ‘Structure, dynamic, function and expression of broad spectrum β-lactamases’, Faculty of Medicine, University of Paris-Sud, Le Kremlin-Bicêtre, France - Department of Bacteriology-Parasitology-Hygiene, Hôpital de Bicêtre, Assistance Publique - Hôpitaux de Paris, Le Kremlin-Bicêtre, France
  • Poirel, Laurent Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland
  • Nordmann, Patrice Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, Department of Medicine, University of Fribourg, Switzerland - Hôpital Cantonal, Fribourg, Switzerland
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    09.08.2015
Published in:
  • Journal of Antimicrobial Chemotherapy. - 2015, vol. 70, no. 11, p. 3014-3022
English The objective of this study was the evaluation of the performance of two commercially available biochemical tests for the rapid detection of carbapenemase-producing Enterobacteriaceae compared with a home-made technique.Methods: A collection of 150 enterobacterial isolates, including 132 isolates with decreased susceptibility to at least one carbapenem molecule, were tested for carbapenemase activity using the RAPIDEC® CARBA NP (bioMérieux), the Rapid CARB Screen® (Rosco Diagnostica) and the home-made Carba NP test. This strain collection included 55 non-carbapenemase producers, 21 KPC producers, 21 NDM producers, 17 VIM producers, 11 IMP producers, 16 OXA-48 producers and 9 OXA-48-like producers (OXA-162, OXA-181, OXA-204, OXA-232 and OXA-244).Results: The RAPIDEC® CARBA NP detected all carbapenemase producers except a single OXA-244 producer. Using the Rapid CARB Screen®, one KPC-2, two NDM-1, one OXA-48 and five OXA-48 variant producers gave equivocal results and one OXA-244 producer was not detected. Using the Carba NP test, the same OXA-244 producer was not detected and one OXA-181 producer and one OXA-244 producer gave equivocal results. Sensitivity and specificity were 99% (95% CI 94.3%–99.8%) and 100% (95% CI 93.5%–100%), respectively, for the RAPIDEC® CARBA NP test, 89.5% (95% CI 81.7%–94.2%) and 70.9% (95% CI 57.9%–81.2%) for the Rapid CARB Screen® and 96.8% (95% CI 91.1%–98.9%) and 100% (95% CI 93.5%–100%) for the Carba NP test. The impact of the use of an adequate bacterial inoculum for obtaining the optimal performance with the RAPIDEC® CARBA NP was noted.Conclusions: The RAPIDEC® CARBA NP possesses the best performance for rapid and efficient detection of carbapenemase-producing Enterobacteriaceae.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304587
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