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NKCC1 downregulation induces hyperpolarizing shift of GABA responsiveness at near term fetal stages in rat cultured dorsal root ganglion neurons

  • Chabwine, Joelle N. Department of Cellular and Molecular Medicine, Katholieke Universiteit Leuven, Louvain, Belgium - Neurology Unit, Department of Medicine, Faculty of Sciences, University of Fribourg, Switzerland
  • Talavera, Karel Laboratory of Ion Channel Research and TRP Channel Research Platform (TRPLe), Department of Cellular and Molecular Medicine, Louvain, Belgium
  • Bosch, Ludo Van Den Laboratory of Neurobiology, Experimental Neurology and Leuven Research Institute for Neuroscience and Disease, Louvain, Belgium - VIB, Vesalius Research Center, Louvain, Belgium
  • Callewaert, Geert Department of Cellular and Molecular Medicine, Katholieke Universiteit Leuven, Louvain, Belgium
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    14.07.2015
Published in:
  • BMC Neuroscience. - 2015, vol. 16, no. 1, p. 41
Dorsal root ganglion neurons
GABA
Intracellular chloride
NKCC1
Bumetanide
Oxytocin
Fetal analgesia
English GABA A receptor-mediated neurotransmission is greatly influenced by cation-chloride cotransporter activity during developmental stages. In embryonic neurons Na–K–2Cl (NKCC1) cotransporters mediate active chloride uptake, thus increasing the intracellular chloride concentration associated with GABA-induced depolarization. At fetal stages near term, oxytocin-induced NKCC1 downregulation has been implicated in the developmental shift from depolarizing to hyperpolarizing GABA action. Mature dorsal root ganglion neurons (DRGN), however, express high NKCC1 levels and maintain high intracellular chloride levels with consequent GABA-induced depolarization.Results: Gramicidin-perforated patch-clamp recordings were used to assess the developmental change in chloride homeostasis in rat cultured small DRGN at the embryonic day 16 (E16) and 19 (E19). The results were compared to data previously obtained in fetal DRGN at E14 and in mature cells. A significant NKCC1 downregulation, leading to reduction in excitatory GABAergic transmission, was observed at E16 and E19.Conclusion: These results indicate that NKCC1 activity transiently decreases in DRGN at fetal stages near term. This developmental shift in GABAergic transmission may contribute to fetal analgesia and neuroprotection at birth.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304536
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