Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract
- Kuhn, Dagmar A. Adolphe Merkle Institute, Université de Fribourg, Switzerland
- Hartmann, Raimo Department of Physics, Philipps Universität Marburg, Marburg, Germany
- Fytianos, Kleanthis Adolphe Merkle Institute, Université de Fribourg, Switzerland
- Petri-Fink, Alke Adolphe Merkle Institute, Université de Fribourg, Switzerland - Chemistry Department, University of Fribourg, Switzerland
- Rothen-Rutishauser, Barbara Adolphe Merkle Institute, Université de Fribourg, Switzerland
- Parak, Wolfgang J. Department of Physics, Philipps Universität Marburg, Marburg, Germany - CIC Biomagune, San Sebastian, Spain
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01.06.2015
Published in:
- Science and Technology of Advanced Materials. - 2015, vol. 16, no. 3, p. 034608
English
Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from dendritic cells by a monolayer of epithelial cells. While passing of small nanoparticles has been demonstrated from macrophages to dendritic cells across the epithelial barrier in previous studies, for the micrometer-sized capsules, this process could not be observed in a significant amount. Thus, this barrier is a limiting factor for cell-to-cell transfer of micrometer-sized particles.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie
- Language
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- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 257151
- DOI 10.1088/1468-6996/16/3/034608
- Persistent URL
- https://folia.unifr.ch/unifr/documents/304491
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