Journal article

Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract

  • Kuhn, Dagmar A. Adolphe Merkle Institute, Université de Fribourg, Switzerland
  • Hartmann, Raimo Department of Physics, Philipps Universität Marburg, Marburg, Germany
  • Fytianos, Kleanthis Adolphe Merkle Institute, Université de Fribourg, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute, Université de Fribourg, Switzerland - Chemistry Department, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute, Université de Fribourg, Switzerland
  • Parak, Wolfgang J. Department of Physics, Philipps Universität Marburg, Marburg, Germany - CIC Biomagune, San Sebastian, Spain
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    01.06.2015
Published in:
  • Science and Technology of Advanced Materials. - 2015, vol. 16, no. 3, p. 034608
English Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from dendritic cells by a monolayer of epithelial cells. While passing of small nanoparticles has been demonstrated from macrophages to dendritic cells across the epithelial barrier in previous studies, for the micrometer-sized capsules, this process could not be observed in a significant amount. Thus, this barrier is a limiting factor for cell-to-cell transfer of micrometer-sized particles.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304491
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