Journal article

Avoiding drying-artifacts in transmission electron microscopy: Characterizing the size and colloidal state of nanoparticles

  • Michen, Benjamin Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Geers, Christoph Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Endes, Carola Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Balog, Sandor Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Chemistry Department, University of Fribourg, Switzerland
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    12.05.2015
Published in:
  • Scientific Reports. - 2015, vol. 5, p. 9793
English Standard transmission electron microscopy nanoparticle sample preparation generally requires the complete removal of the suspending liquid. Drying often introduces artifacts, which can obscure the state of the dispersion prior to drying and preclude automated image analysis typically used to obtain number-weighted particle size distribution. Here we present a straightforward protocol for prevention of the onset of drying artifacts, thereby allowing the preservation of in-situ colloidal features of nanoparticles during TEM sample preparation. This is achieved by adding a suitable macromolecular agent to the suspension. Both research- and economically-relevant particles with high polydispersity and/or shape anisotropy are easily characterized following our approach (http://bsa.bionanomaterials.ch), which allows for rapid and quantitative classification in terms of dimensionality and size: features that are major targets of European Union recommendations and legislation.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304422
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