Localization and expression of EDS5H a homologue of the SA transporter EDS5

Parinthawong, Nonglak; Cottier, Stéphanie; Buchala, Antony J.; Nawrath, Christiane; Métraux, Jean-Pierre

doi:10.1186/s12870-015-0518-1

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Localization and expression of EDS5H a homologue of the SA transporter EDS5

Parinthawong, Nonglak Department of Biology, University of Fribourg, Switzerland - Faculty of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand
Cottier, Stéphanie Department of Biology, University of Fribourg, Switzerland
Buchala, Antony J. Department of Biology, University of Fribourg, Switzerland
Nawrath, Christiane Department of Biology, University of Fribourg, Switzerland - Department of Plant Molecular Biology, University of Lausanne, Switzerland
Métraux, Jean-Pierre Department of Biology, University of Fribourg, Switzerland

09.06.2015

Published in:

BMC Plant Biology. - 2015, vol. 15, no. 1, p. 135

English An important signal transduction pathway in plant defence depends on the accumulation of salicylic acid (SA). SA is produced in chloroplasts and the multidrug and toxin extrusion transporter ENHANCED DISEASE SUSCEPTIBILITY5 (EDS5; At4g39030) is necessary for the accumulation of SA after pathogen and abiotic stress. EDS5 is localized at the chloroplast and functions in transporting SA from the chloroplast to the cytoplasm. EDS5 has a homologue called EDS5H (EDS5 HOMOLOGUE; At2g21340) but its relationship to EDS5 has not been described and its function is not known. Results: EDS5H exhibits about 72 % similarity and 59 % identity to EDS5. In contrast to EDS5 that is induced after pathogen inoculation, EDS5H was constitutively expressed in all green tissues, independently of pathogen infection. Both transporters are located at the envelope of the chloroplast, the compartment of SA biosynthesis. EDS5H is not involved with the accumulation of SA after inoculation with a pathogen or exposure to UV stress. A phylogenetic analysis supports the hypothesis that EDS5H may be an H+/organic acid antiporter like EDS5. Conclusions: The data based on genetic and molecular studies indicate that EDS5H despite its homology to EDS5 does not contribute to pathogen-induced SA accumulation like EDS5. EDS5H most likely transports related substances such as for example phenolic acids, but unlikely SA.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language