Journal article
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Complementation of the embryo-lethal T-DNA insertion mutant of AUXIN-BINDING-PROTEIN 1 (ABP1) with abp1 point mutated versions reveals crosstalk of ABP1 and phytochromes
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Effendi, Yunus
Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Hannover, Germany - Al Azhar Indonesia University, Department of Biology, Jakarta, Indonesia
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Ferro, Noel
University of Bonn, Mulliken Center for Theoretical Chemistry, Institute for Physical and Theoretical Chemistry, Bonn, Germany
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Labusch, Corinna
Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Hannover, Germany
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Geisler, Markus
University of Fribourg, Department of Biology - Plant Biology, Switzerland
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Scherer, Günther F. E.
Leibniz Universität Hannover, Institut für Gartenbauliche Produktionssysteme, Abt. Molekulare Ertragsphysiologie, Hannover, Germany
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Published in:
- Journal of Experimental Botany. - 2015, vol. 66, no. 1, p. 403-418
English
The function of the extracytoplasmic AUXIN-BINDING-PROTEIN1 (ABP1) is largely enigmatic. We complemented a homozygous T-DNA insertion null mutant of ABP1 in Arabidopsis thaliana Wassilewskia with three mutated and one wild-type (wt) ABP1 cDNA, all tagged C-terminally with a strepII–FLAG tag upstream the KDEL signal. Based on in silico modelling, the abp1 mutants were predicted to have altered geometries of the auxin binding pocket and calculated auxin binding energies lower than the wt. Phenotypes linked to auxin transport were compromised in these three complemented abp1 mutants. Red light effects, such as elongation of hypocotyls in constant red (R) and far-red (FR) light, in white light supplemented by FR light simulating shade, and inhibition of gravitropism by R or FR, were all compromised in the complemented lines. Using auxin- or light-induced expression of marker genes, we showed that auxin-induced expression was delayed already after 10min, and light-induced expression within 60min, even though TIR1/AFB or phyB are thought to act as receptors relevant for gene expression regulation. The expression of marker genes in seedlings responding to both auxin and shade showed that for both stimuli regulation of marker gene expression was altered after 10–20min in the wild type and phyB mutant. The rapidity of expression responses provides a framework for the mechanics of functional interaction of ABP1 and phyB to trigger interwoven signalling pathways.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Biologie
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Language
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Classification
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Biological sciences
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/304298
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