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Integrating silver compounds and nanoparticles into ceria nanocontainers for antimicrobial applications

  • Gagnon, Jacinthe Department of Chemistry and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Clift, Martin J. D. Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Vanhecke, Dimitri Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Kuhn, Dagmar Alice Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Weber, Pierric Department of Chemistry and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Fromm, Katharina M. Department of Chemistry and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
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    18.02.2015
Published in:
  • Journal of Materials Chemistry B. - 2015, vol. 3, no. 9, p. 1760–1768
English Silver compounds and nanoparticles (NPs) are gaining increasing interest in medical applications, specifically in the treatment and prevention of biomaterial-related infections. However, the silver release from these materials, resulting in a limited antimicrobial activity, is often difficult to control. In this paper, ceria nanocontainers were synthesized by a template-assisted method and were then used to encapsulate silver nitrate (AgNO₃/CeO₂ nanocontainers). Over the first 30 days, a significant level of silver was released, as determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). A novel type of ceria container containing silver NPs (AgNP/CeO₂ containers) was also developed using two different template removal methods. The presence of AgNPs was confirmed both on the surface and in the interior of the ceria containers by X-ray diffraction (XRD), transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Upon removal of the template by calcination, the silver was released over a period exceeding three months (>90 days). However, when the template was removed by dissolution, the silver release was shortened to ≤14 days. The antimicrobial activity of the silver-containing CeO₂ containers was observed and the minimum bactericidal concentration (MBC) was determined using the broth dilution method. Investigation on human cells, using a model epithelial barrier cell type (A549 cells), highlighted that all three samples induced a heightened cytotoxicity leading to cell death when exposed to all containers in their raw form. This was attributed to the surface roughness of the CeO₂ nanocontainers and the kinetics of the silver release from the AgNO₃/CeO₂ and AgNP/CeO₂ nanocontainers. In conclusion, despite the need for further emphasis on their biocompatibility, the concept of the AgNP/CeO₂ nanocontainers offers a potentially alternative long-term antibactericidal strategy for implant materials.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304296
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