Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro
- Fytianos, Kleanthis Adolphe Merkle Institute, University of Fribourg, Switzerland
- Rodriguez-Lorenzo, Laura Adolphe Merkle Institute, University of Fribourg, Switzerland
- Clift, Martin J. D. Adolphe Merkle Institute, University of Fribourg, Switzerland
- Blank, Fabian Respiratory Medicine, Bern University Hospital, Bern, Switzerland
- Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
- Garnier, Christophe von Respiratory Medicine, Bern University Hospital, Bern, Switzerland
- Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
- Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland - Respiratory Medicine, Bern University Hospital, Bern, Switzerland
-
2015
Published in:
- Nanomedicine: Nanotechnology, Biology and Medicine. - 2015, vol. 11, no. 3, p. 633–644
English
Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie
- Language
-
- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 255803
- DOI 10.1016/j.nano.2014.11.004
- Persistent URL
- https://folia.unifr.ch/unifr/documents/304256
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