Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Fytianos, Kleanthis; Rodriguez-Lorenzo, Laura; Clift, Martin J. D.; Blank, Fabian; Vanhecke, Dimitri; Garnier, Christophe von; Petri-Fink, Alke; Rothen-Rutishauser, Barbara

doi:10.1016/j.nano.2014.11.004

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Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

Fytianos, Kleanthis Adolphe Merkle Institute, University of Fribourg, Switzerland
Rodriguez-Lorenzo, Laura Adolphe Merkle Institute, University of Fribourg, Switzerland
Clift, Martin J. D. Adolphe Merkle Institute, University of Fribourg, Switzerland
Blank, Fabian Respiratory Medicine, Bern University Hospital, Bern, Switzerland
Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
Garnier, Christophe von Respiratory Medicine, Bern University Hospital, Bern, Switzerland
Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland - Respiratory Medicine, Bern University Hospital, Bern, Switzerland

2015

Published in:

Nanomedicine: Nanotechnology, Biology and Medicine. - 2015, vol. 11, no. 3, p. 633–644

English Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie

Language