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Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro

  • Fytianos, Kleanthis Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Rodriguez-Lorenzo, Laura Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Clift, Martin J. D. Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Blank, Fabian Respiratory Medicine, Bern University Hospital, Bern, Switzerland
  • Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Garnier, Christophe von Respiratory Medicine, Bern University Hospital, Bern, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland - Respiratory Medicine, Bern University Hospital, Bern, Switzerland
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    2015
Published in:
  • Nanomedicine: Nanotechnology, Biology and Medicine. - 2015, vol. 11, no. 3, p. 633–644
English Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/304256
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