Journal article
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Uptake efficiency of surface modified gold nanoparticles does not correlate with functional changes and cytokine secretion in human dendritic cells in vitro
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Fytianos, Kleanthis
Adolphe Merkle Institute, University of Fribourg, Switzerland
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Rodriguez-Lorenzo, Laura
Adolphe Merkle Institute, University of Fribourg, Switzerland
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Clift, Martin J. D.
Adolphe Merkle Institute, University of Fribourg, Switzerland
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Blank, Fabian
Respiratory Medicine, Bern University Hospital, Bern, Switzerland
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Vanhecke, Dimitri
Adolphe Merkle Institute, University of Fribourg, Switzerland
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Garnier, Christophe von
Respiratory Medicine, Bern University Hospital, Bern, Switzerland
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Petri-Fink, Alke
Adolphe Merkle Institute, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
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Rothen-Rutishauser, Barbara
Adolphe Merkle Institute, University of Fribourg, Switzerland - Respiratory Medicine, Bern University Hospital, Bern, Switzerland
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Published in:
- Nanomedicine: Nanotechnology, Biology and Medicine. - 2015, vol. 11, no. 3, p. 633–644
English
Engineering nanoparticles (NPs) for immune modulation require a thorough understanding of their interaction(s) with cells. Gold NPs (AuNPs) were coated with polyethylene glycol (PEG), polyvinyl alcohol (PVA) or a mixture of both with either positive or negative surface charge to investigate uptake and cell response in monocyte-derived dendritic cells (MDDCs). Inductively coupled plasma optical emission spectrometry and transmission electron microscopy were used to confirm the presence of Au inside MDDCs. Cell viability, (pro-)inflammatory responses, MDDC phenotype, activation markers, antigen uptake and processing were analyzed. Cell death was only observed for PVA-NH2 AuNPs at the highest concentration. MDDCs internalize AuNPs, however, surface modification influenced uptake. Though limited uptake was observed for PEG-COOH AuNPs, a significant tumor necrosis factor-alpha release was induced. In contrast, (PEG + PVA)-NH2 and PVA-NH2 AuNPs were internalized to a higher extent and caused interleukin-1beta secretion. None of the AuNPs caused changes in MDDC phenotype, activation or immunological properties.
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Faculty
- Faculté des sciences et de médecine
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Department
- Département de Chimie
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Language
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Classification
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Chemistry
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License
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License undefined
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Identifiers
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Persistent URL
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https://folia.unifr.ch/unifr/documents/304256
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