Nucleotide excision repair efficiency in quiescent human fibroblasts is modulated by circadian clock
- Bee, Leonardo Department of Biology, University of Padova, Italy
- Marini, Selena Department of Biology, University of Padova, Italy
- Pontarin, Giovanna Department of Biology, University of Padova, Italy
- Ferraro, Paola Department of Biology, University of Padova, Italy
- Costa, Rodolfo Department of Biology, University of Padova, Italy
- Albrecht, Urs Department of Biology, Unit for Biochemistry, University of Fribourg, Switzerland
- Celotti, Lucia Department of Biology, University of Padova, Italy
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27.02.2015
Published in:
- Nucleic Acids Research. - 2015, vol. 43, no. 4, p. 2126-2137
English
The efficiency of Nucleotide Excision Repair (NER)process is crucial for maintaining genomic integrity because in many organisms, including humans, it represents the only system able to repair a wide range of DNA damage. The aim of the work was to investigate whether the efficiency of the repair of photoproducts induced by UV-light is affected by the circadian phase at which irradiation occurred. NER activity has been analyzed in human quiescent fibroblasts (in the absence of the cell cycle effect), in which circadian rhythmicity has been synchronized with a pulse of dexamethasone. Our results demonstrate that both DNA damage induction and repair efficiency are strictly dependent on the phase of the circadian rhythm at which the cells are UV-exposed. Furthermore, the differences observed between fibroblasts irradiated at different circadian times (CTs) are abolished when the clock is obliterated. In addition, we observe that chromatin structure is regulated by circadian rhythmicity. Maximal chromatin relaxation occurred at the same CT when photoproduct formation and removal were highest. Our data suggest that the circadian clock regulates both the DNA sensitivity to UV damage and the efficiency of NER by controlling chromatin condensation mainly through histone acetylation.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 234833
- DOI 10.1093/nar/gkv081
- Persistent URL
- https://folia.unifr.ch/unifr/documents/304137
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