Journal article

Comprehensive transcriptome analysis unravels the existence of crucial genes regulating primary metabolism during adventitious root formation in Petunia hybrida

  • Ahkami, Amirhossein Institute of Biological Chemistry, Washington State University, Pullman, USA
  • Scholz, Uwe Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
  • Steuernagel, Burkhard The Sainsbury Laboratory, Norwich Research Park, Norwich, UK
  • Strickert, Marc SYNMIKRO, Philipps-Universität, Marburg, Germany
  • Haensch, Klaus-Thomas Leibniz Institute of Vegetable and Ornamental Crops (IGZ), Grossbeeren & Erfurt, Germany
  • Druege, Uwe Leibniz Institute of Vegetable and Ornamental Crops (IGZ), Grossbeeren & Erfurt, Germany
  • Reinhardt, Didier Department of Biology, University of Fribourg, Switzerland
  • Nouri, Eva Department of Biology, University of Fribourg, Switzerland
  • Wirén, Nicolaus von Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
  • Franken, Philipp Leibniz Institute of Vegetable and Ornamental Crops (IGZ), Grossbeeren & Erfurt, Germany
  • Hajirezaei, Mohammad-Reza Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany
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    30.06.2014
Published in:
  • PLoS ONE. - 2014, vol. 9, no. 6, p. e100997
English To identify specific genes determining the initiation and formation of adventitious roots (AR), a microarray-based transcriptome analysis in the stem base of the cuttings of Petunia hybrida (line W115) was conducted. A microarray carrying 24,816 unique, non-redundant annotated sequences was hybridized to probes derived from different stages of AR formation. After exclusion of wound-responsive and root-regulated genes, 1,354 of them were identified which were significantly and specifically induced during various phases of AR formation. Based on a recent physiological model distinguishing three metabolic phases in AR formation, the present paper focuses on the response of genes related to particular metabolic pathways. Key genes involved in primary carbohydrate metabolism such as those mediating apoplastic sucrose unloading were induced at the early sink establishment phase of AR formation. Transcriptome changes also pointed to a possible role of trehalose metabolism and SnRK1 (sucrose non-fermenting 1- related protein kinase) in sugar sensing during this early step of AR formation. Symplastic sucrose unloading and nucleotide biosynthesis were the major processes induced during the later recovery and maintenance phases. Moreover, transcripts involved in peroxisomal beta-oxidation were up-regulated during different phases of AR formation. In addition to metabolic pathways, the analysis revealed the activation of cell division at the two later phases and in particular the induction of G1- specific genes in the maintenance phase. Furthermore, results point towards a specific demand for certain mineral nutrients starting in the recovery phase.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/303767
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