TORC1 regulates Pah1 phosphatidate phosphatase activity via the Nem1/Spo7 protein phosphatase complex
-
Dubots, Emmanuelle
Department of Biology, University of Fribourg, Switzerland
-
Cottier, Stéphanie
Department of Biology, University of Fribourg, Switzerland
-
Péli-Gulli, Marie-Pierre
Department of Biology, University of Fribourg, Switzerland
-
Jaquenoud, Malika
Department of Biology, University of Fribourg, Switzerland
-
Bontron, Séverine
Department of Biology, University of Fribourg, Switzerland
-
Schneiter, Roger
Department of Biology, University of Fribourg, Switzerland
-
De Virgilio, Claudio
Department of Biology, University of Fribourg, Switzerland
Show more…
Published in:
- PLoS ONE. - 2014, vol. 9, no. 8, p. e104194
English
The evolutionarily conserved target of rapamycin complex 1 (TORC1) controls growth-related processes such as protein, nucleotide, and lipid metabolism in response to growth hormones, energy/ATP levels, and amino acids. Its deregulation is associated with cancer, type 2 diabetes, and obesity. Among other substrates, mammalian TORC1 directly phosphorylates and inhibits the phosphatidate phosphatase lipin-1, a central enzyme in lipid metabolism that provides diacylglycerol for the synthesis of membrane phospholipids and/or triacylglycerol as neutral lipid reserve. Here, we show that yeast TORC1 inhibits the function of the respective lipin, Pah1, to prevent the accumulation of triacylglycerol. Surprisingly, TORC1 regulates Pah1 in part indirectly by controlling the phosphorylation status of Nem1 within the Pah1-activating, heterodimeric Nem1-Spo7 protein phosphatase module. Our results delineate a hitherto unknown TORC1 effector branch that controls lipin function in yeast, which, given the recent discovery of Nem1-Spo7 orthologous proteins in humans, may be conserved.
-
Faculty
- Faculté des sciences et de médecine
-
Department
- Département de Biologie
-
Language
-
-
Classification
-
Biological sciences
-
License
-
License undefined
-
Identifiers
-
-
Persistent URL
-
https://folia.unifr.ch/unifr/documents/303724
Statistics
Document views: 50
File downloads: