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Rapid detection of extended-spectrum-β-lactamase-producing Enterobacteriaceae from urine samples by use of the ESBL NDP test

  • Dortet, Laurent INSERM U914, Le Kremlin-Bicêtre, France - Centre National Associé-Centre de Référence des Résistances aux Antibiotiqus, Le Kremlin-Bicêtre, France
  • Poirel, Laurent INSERM U914, Le Kremlin-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
  • Nordmann, Patrice INSERM U914, Le Kremlin-Bicêtre, France - Centre National Associé-Centre de Référence des Résistances aux Antibiotiqus, Le Kremlin-Bicêtre, France - Medical and Molecular Microbiology Unit, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland - Hôpital Cantonal, Fribourg, Switzerland
    10.01.2014
Published in:
  • Journal of Clinical Microbiology. - 2014, vol. 52, no. 10, p. 3701–3706
English From June to September 2012, 500 urine samples were recovered from patients with urinary tract infections (UTI) due to Gram-negative bacilli (≥10⁴ leukocytes/ml and ≥10⁵ Gram-negative isolates/ml) who visited the University hospital Bicêtre (France). They were challenged with extended-spectrum-β-lactamase (ESBL)-producing Enterobacteriaceae (ESBL-E) using the rapid diagnostic ESBL NDP test. Results of the ESBL NDP test were compared to the results of the double-disc susceptibility test (DDST) performed on solid-agar plates and molecular identification of the β-lactamase genes. Among the 450 nonduplicate urine samples, 11.3% were positive for ESBL-E by using the DDST, the ESBL determinants being mostly of the CTX-M type (CTX-M-15) according to molecular testing. Results of the ESBL NDP test were obtained within 15 min. The sensitivity and specificity of the ESBL NDP test were 98% and 99.8%, respectively, whereas the positive and negative predictive values of this test were 98% and 99.8%, respectively. A perfect correlation between cefotaxime resistance and positivity of the ESBL NDP test was observed. Therefore, the ESBL NDP test offers a powerful tool for a rapid identification of ESBL-E and associated resistance to expanded-spectrum cephalosporins. It may be useful in particular for guiding first-line antibiotic therapy.
Faculty
Faculté des sciences et de médecine
Department
Médecine 3ème année
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/303690
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