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Expression of a microbial serine proteinase inhibitor gene enhances the tobacco defense against oomycete pathogens

  • Silva, Yussuan Center for Genetic Engineering and Biotechnology, Havana, Cuba - Tobacco Research Institute, San Antonio de los Baños, Havana, Cuba
  • Portieles, Roxana Center for Genetic Engineering and Biotechnology, Havana, Cuba
  • Pujol, Merardo Center for Genetic Engineering and Biotechnology, Havana, Cuba
  • Terauchi, Ryohei Iwate Biotechnology Research Center, Kitakami, Iwate, Japan
  • Matsumura, Hideo Gene Research Center, Shinshu University, Ueda, Japan
  • Serrano, Mario Department of Biology, University of Fribourg, Fribourg, Switzerland
  • Borrás-Hidalgo, Orlando Center for Genetic Engineering and Biotechnology, Havana, Cuba
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    15.08.2013
Published in:
  • Physiological and Molecular Plant Pathology. - 2013, vol. 84, p. 99–106
English In order to identify Nicotiana megalosiphon genes for novel inhibitors of microbial serine proteinase involved in resistance to the oomycete Phytophthora parasitica var. nicotianae, SuperSAGE technology combined with next-generation sequencing were used to generate libraries in order to identify transcripts that are differentially up-regulated. We identified a N. megalosiphon inhibitor of microbial serine proteinase gene (NmIMSP) rapidly induced during the interaction. Silencing of NmIMSP gene was found to compromise the resistance of N. megalosiphon to P. parasitica var. nicotianae. Herewith, the transient expression of NmIMSP gene in a susceptible genotype conferred high level of resistance to P. parasitica var. nicotianae and Peronospora hyoscyami f. sp. tabacina. This is the first evidence of this inhibitor of microbial serine proteinase as a positive regulator of defense response against oomycete.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/303524
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