Journal article
+ 1 other files
Expression of a microbial serine proteinase inhibitor gene enhances the tobacco defense against oomycete pathogens
-
Silva, Yussuan
Center for Genetic Engineering and Biotechnology, Havana, Cuba - Tobacco Research Institute, San Antonio de los Baños, Havana, Cuba
-
Portieles, Roxana
Center for Genetic Engineering and Biotechnology, Havana, Cuba
-
Pujol, Merardo
Center for Genetic Engineering and Biotechnology, Havana, Cuba
-
Terauchi, Ryohei
Iwate Biotechnology Research Center, Kitakami, Iwate, Japan
-
Matsumura, Hideo
Gene Research Center, Shinshu University, Ueda, Japan
-
Serrano, Mario
Department of Biology, University of Fribourg, Fribourg, Switzerland
-
Borrás-Hidalgo, Orlando
Center for Genetic Engineering and Biotechnology, Havana, Cuba
Show more…
Published in:
- Physiological and Molecular Plant Pathology. - 2013, vol. 84, p. 99–106
English
In order to identify Nicotiana megalosiphon genes for novel inhibitors of microbial serine proteinase involved in resistance to the oomycete Phytophthora parasitica var. nicotianae, SuperSAGE technology combined with next-generation sequencing were used to generate libraries in order to identify transcripts that are differentially up-regulated. We identified a N. megalosiphon inhibitor of microbial serine proteinase gene (NmIMSP) rapidly induced during the interaction. Silencing of NmIMSP gene was found to compromise the resistance of N. megalosiphon to P. parasitica var. nicotianae. Herewith, the transient expression of NmIMSP gene in a susceptible genotype conferred high level of resistance to P. parasitica var. nicotianae and Peronospora hyoscyami f. sp. tabacina. This is the first evidence of this inhibitor of microbial serine proteinase as a positive regulator of defense response against oomycete.
-
Faculty
- Faculté des sciences et de médecine
-
Department
- Département de Biologie
-
Language
-
-
Classification
-
Biological sciences
-
License
-
License undefined
-
Identifiers
-
-
Persistent URL
-
https://folia.unifr.ch/unifr/documents/303524
Other files
Statistics
Document views: 30
File downloads:
- serr_ems.pdf: 114
- serr_ems_sm.pdf: 66