Journal article

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Modeling nanoparticle–alveolar epithelial cell interactions under breathing conditions using captive bubble surfactometry

  • Schürch, David Adolphe Merkle Institute, University of Fribourg, Switzerland - Institute of Anatomy, University of Bern, Switzerland
  • Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Clift, Martin J. D. Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Raemy, David Adolphe Merkle Institute, University of Fribourg, Switzerland - Institute of Anatomy, University of Bern, Switzerland
  • Aberasturi, Dorleta Jimenez de Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany
  • Parak, Wolfgang J. Fachbereich Physik, Philipps Universität Marburg, Marburg, Germany
  • Gehr, Peter Institute of Anatomy, University of Bern, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Respiratory Medicine, Bern University Hospital, Switzerland
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    06.05.2014
Published in:
  • Langmuir. - 2014, vol. 30, no. 17, p. 4924–4932
English Many advances have been made in recent years in cell culture models of the epithelial barrier of the lung from simple monolayers to complex 3-D systems employing different cell types. However, the vast majority of these models still present a static air–liquid interface which is unrealistic given the dynamic nature of breathing. We present here a method where epithelial lung cells are integrated into a system, the captive bubble surfactometer, which allows the cyclical compression and expansion of the surfactant film at the air–liquid interface, thus modeling the dynamics of breathing. We found that cellular uptake of deposited gold nanoparticles was significantly increased under the dynamic (breathing) conditions of compression and expansion as compared to static conditions. The method could be very useful for studying nanoparticle–alveolar lung cell interactions under breathing conditions for applications in nanomedicine and toxicology.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/303495
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