Insertion of Nanoparticle Clusters into Vesicle Bilayers

Bonnaud, Cécile; Monnier, Christophe A.; Demurtas, Davide; Jud, Corinne; Vanhecke, Dimitri; Montet, Xavier; Hovius, Ruud; Lattuada, Marco; Rothen-Rutishauser, Barbara; Petri-Fink, Alke

doi:10.1021/nn406349z

Back

Journal article

+ 1 other files

Insertion of Nanoparticle Clusters into Vesicle Bilayers

Bonnaud, Cécile Adolphe Merkle Institute, University of Fribourg, Switzerland - Chemistry Department, University of Fribourg, Switzerland
Monnier, Christophe A. Adolphe Merkle Institute, University of Fribourg, Switzerland
Demurtas, Davide Interdisciplinary Center for Electron Microscopy, Ecole Polytechnique Fédérale de Lausanne, Switzerland
Jud, Corinne Adolphe Merkle Institute, University of Fribourg, Switzerland
Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Switzerland
Montet, Xavier Radiology Department, Geneva University Hospital, Rue Gabrielle-Perret-Gentil 4, 1211 Genève 14, Switzerland
Hovius, Ruud Laboratory of Physical Chemistry of Polymers and Membranes, Ecole Polytechnique Fédérale de Lausanne, Station 6, 1015 Lausanne, Switzerland
Lattuada, Marco Adolphe Merkle Institute, University of Fribourg, Switzerland
Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Switzerland - Respiratory Medicine, Bern University Hospital, Inselspital, Freiburgstrasse, 3010 Bern, Switzerland
Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Switzerland - Chemistry Department, University of Fribourg, Switzerland

22.04.2014

Published in:

ACS Nano. - 2014, vol. 8, no. 4, p. 3451–3460

English A major contemporary concern in developing effective liposome–nanoparticle hybrids is the present inclusion size limitation of nanoparticles between vesicle bilayers, which is considered to be around 6.5 nm in diameter. In this article, we present experimental observations backed by theoretical considerations which show that greater structures can be incorporated within vesicle membranes by promoting the clustering of nanoparticles before liposome formation. Cryo-transmission electron microscopy and cryo-electron tomography confirm these observations at unprecedented detail and underpin that the liposome membranes can accommodate flexible structures of up to 60 nm in size. These results imply that this material is more versatile in terms of inclusion capabilities and consequently widens the opportunities in developing multivalent vesicles for nanobiotechnology applications.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie

Language