Cell-Permeant and photocleavable chemical inducer of dimerization
- Zimmermann, Mirjam University of Basel, Department of Biomedicine, Switzerland
- Cal, Ruben University of Basel, Department of Biomedicine, Switzerland
- Janett, Elia University of Fribourg, Department of Chemistry, Switzerland
- Hoffmann, Viktor University of Basel, Department of Biomedicine, Switzerland
- Bochet, Christian G. University of Fribourg, Department of Chemistry, Switzerland
- Constable, Edwin University of Basel, Department of Chemistry, Switzerland
- Beaufils, Florent University of Basel, Department of Biomedicine, Switzerland
- Wymann, Matthias P. University of Basel, Department of Biomedicine, Switzerland
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25.04.2014
Published in:
- Angewandte Chemie International Edition. - 2014, vol. 53, no. 18, p. 4717–4720
chemical inducers of dimerization
fusion proteins
photocleavable linkers
photolysis
protein protein interactions
English
Chemical inducers of dimerization (CIDs) have been developed to orchestrate protein dimerization and translocation. Here we present a novel photocleavable HaloTag- and SNAP-tag-reactive CID (MeNV-HaXS) with excellent selectivity and intracellular reactivity. Excitation at 360 nm cleaves the methyl-6-nitroveratryl core of MeNV-HaXS. MeNV-HaXS covalently links HaloTag- and SNAP-tag fusion proteins, and enables targeting of selected membranes and intracellular organelles. MeNV-HaXS-mediated translocation has been validated for plasma membrane, late endosomes, lysosomes, Golgi, mitochondria, and the actin cytoskeleton. Photocleavage of MeNV-HaXS liberates target proteins and provides access to optical manipulation of protein relocation with high spatiotemporal and subcellular precision. MeNV-HaXS supports kinetic studies of protein dynamics and the manipulation of subcellular enzyme activities, which is exemplified for Golgi-targeted cargo and the assessment of nuclear import kinetics.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Chimie
- Language
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- English
- Classification
- Chemistry
- License
- License undefined
- Identifiers
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- RERO DOC 210015
- DOI 10.1002/anie.201310969
- Persistent URL
- https://folia.unifr.ch/unifr/documents/303459
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