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Can the Ames test provide an insight into nano-object mutagenicity? Investigating the interaction between nano-objects and bacteria

  • Clift, Martin J. D. Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Raemy, David O. Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Endes, Carola Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Ali, Zulqurnain Department of Physics, Philipps University Marburg, Germany
  • Lehmann, Andrea D. Institute of Anatomy, University of Bern, Switzerland
  • Brandenberger, Christina Institute of Anatomy, University of Bern, Switzerland
  • Petri-Fink, Alke Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Wick, Peter Empa, Materials-Biology Interactions Laboratory, St. Gallen, Switzerland
  • Parak, Wolfgang J. Department of Physics, Philipps University Marburg, Germany
  • Gehr, Peter Institute of Anatomy, University of Bern, Switzerland
  • Schins, Roel P. F. IUF - Leibniz Research Institute for Environmental Medicine, Duesseldorf, Germany
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland - Respiratory Medicine, Bern University Hospital, Bern, Switzerland
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    2013
Published in:
  • Nanotoxicology. - 2013, vol. 7, no. 8, p. 1373–1385
nano-object(s)
nano-bacterial interactions
mutagenicity
Ames test
mammalian cells
English The aim of this study was to assess the interaction of a series of well characterised nano-objects with the Gram negative bacterium Salmonella typhimurium, and how such an interaction may relate to the potential mutagenicity of nano-objects. Transmission electron microscopy showed that nano-objects (Au-PMA-ATTO NPs, CeO₂ NPs, SWCNTs and MWCNTs), as well as CAFs entered S. typhimurium. Only DEPs did not penetrate/enter the bacteria, however, were the only particle stimulus to induce any significant mutagenicity through the Ames test. Comparison with a sophisticated 3D in vitro cell model showed CAFs, DEPs, SWCNTs and MWCNTs to cause a significant increase in mammalian cell proliferation, whilst both the Au-PMA-ATTO NPs and CeO₂ NPs had not significant adverse effects. In conclusion, these results indicate that various of different nano-objects are able to penetrate the double-lipid bilayer of Gram negative bacteria, although the Ames test may not be a good indicator for nano-object mutagenicity.
Faculty
Faculté des sciences et de médecine
Department
AMI - Bio-Nanomatériaux
Language
  • English
Classification
Biological sciences
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/303314
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