Journal article

Exposure of silver-nanoparticles and silver-ions to lung cells in vitro at the air-liquid interface

  • Herzog, Fabian Adolphe Merkle Institute, Bio-Nanomaterials, University of Fribourg, Switzerland
  • Clift, Martin J. D. Adolphe Merkle Institute, Bio-Nanomaterials, University of Fribourg, Switzerland
  • Piccapietra, Flavio Eawag ,Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland
  • Behra, Renata Eawag ,Swiss Federal Institute of Aquatic Science and Technology, Dübendorf, Switzerland
  • Schmid, Otmar Comprehensive Pneumology Center, Institute of Lung Biology and Disease Helmholtz Zentrum München, Neuherberg, Germany
  • Petri-Fink, Alke Adolphe Merkle Institute, Bio-Nanomaterials, University of Fribourg, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Rothen-Rutishauser, Barbara Adolphe Merkle Institute, Bio-Nanomaterials, University of Fribourg, Switzerland - Respiratory Medicine, Department of Clinical Research, Inselspital University Hospital, University of Bern, Switzerland
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    04.04.2013
Published in:
  • Particle and Fibre Toxicology. - 2013, vol. 10, no. 1, p. 11
English Background Due to its antibacterial properties, silver (Ag) has been used in more consumer products than any other nanomaterial so far. Despite the promising advantages posed by using Ag-nanoparticles (NPs), their interaction with mammalian systems is currently not fully understood. An exposure route via inhalation is of primary concern for humans in an occupational setting. Aim of this study was therefore to investigate the potential adverse effects of aerosolised Ag-NPs using a human epithelial airway barrier model composed of A549, monocyte derived macrophage and dendritic cells cultured in vitro at the air-liquid interface. Cell cultures were exposed to 20 nm citrate-coated Ag-NPs with a deposition of 30 and 278 ng/cm² respectively and incubated for 4 h and 24 h. To elucidate whether any effects of Ag-NPs are due to ionic effects, Ag-Nitrate (AgNO₃) solutions were aerosolised at the same molecular mass concentrations.Results Agglomerates of Ag-NPs were detected at 24 h post exposure in vesicular structures inside cells but the cellular integrity was not impaired upon Ag-NP exposures. Minimal cytotoxicity, by measuring the release of lactate dehydrogenase, could only be detected following a higher concentrated AgNO₃-solution. A release of pro-inflammatory markers TNF-α and IL-8 was neither observed upon Ag-NP and AgNO₃ exposures as well as was not affected when cells were pre-stimulated with lipopolysaccharide (LPS). Also, an induction of mRNA expression of TNF-α and IL-8, could only be observed for the highest AgNO₃ concentration alone or even significantly increased when pre-stimulated with LPS after 4 h. However, this effect disappeared after 24 h. Furthermore, oxidative stress markers (HMOX-1, SOD-1) were expressed after 4 h in a concentration dependent manner following AgNO₃ exposures only.Conclusions With an experimental setup reflecting physiological exposure conditions in the human lung more realistic, the present study indicates that Ag-NPs do not cause adverse effects and cells were only sensitive to high Ag-ion concentrations. Chronic exposure scenarios however, are needed to reveal further insight into the fate of Ag-NPs after deposition and cell interactions.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Medicine
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/303189
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