Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the EGO complex to activate TORC1

Zhang, Tianlong; Péli-Gulli, Marie-Pierre; Yang, Hui; De Virgilio, Claudio; Ding, Jianping

Back

Journal article

Ego3 functions as a homodimer to mediate the interaction between Gtr1-Gtr2 and Ego1 in the EGO complex to activate TORC1

Zhang, Tianlong Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Academy of Sciences, Shanghai, China -
Péli-Gulli, Marie-Pierre Division of Biochemistry, Department of Biology, University of Fribourg, Switzerland -
Yang, Hui Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Academy of Sciences, Shanghai, China
De Virgilio, Claudio Division of Biochemistry, Department of Biology, University of Fribourg, Switzerland
Ding, Jianping Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Academy of Sciences, Shanghai, China

01.11.2012

Published in:

Structure. - 2012, vol. 20, no. 12, p. 2151–2160

English The yeast EGO complex, consisting of Gtr1, Gtr2, Ego1, and Ego3, localizes to the endosomal and vacuolar membranes and plays a pivotal role in cell growth and autophagy regulation through relaying amino acid signals to activate TORC1. Here, we report the crystal structures of a wild-type and a mutant form of Saccharomyces cerevisiae Ego3. Ego3 assumes a homodimeric structure similar to that of the mammalian MP1-p14 heterodimer and the C-terminal domains of the yeast Gtr1-Gtr2 heterodimer, both of which function in TORC1 signaling. Structural and genetic data demonstrate that the unique dimer conformation of Ego3 is essential for the integrity and function of the EGO complex. Structural and functional data also identify a potential binding site for Gtr1-Gtr2. These results suggest a structural conservation of the protein components involved in amino acid signaling to TORC1 and reveal structural insights into the molecular mechanism of Ego3 function in TORC1 signaling.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language