Spatial SPION localization in liposome membranes

Bonnaud, Cécile; Vanhecke, Dimitri; Demurtas, Davide; Rothen-Rutishauser, Barbara; Petri-Fink, Alke

doi:10.1109/TMAG.2012.2219040

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Journal article

Spatial SPION localization in liposome membranes

Bonnaud, Cécile Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland - Chemistry Department, University of Fribourg, Switzerland
Vanhecke, Dimitri Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland
Demurtas, Davide Interdisciplinary Center for Electron Microscopy, École Polytechnique Fédérale de Lausanne, Switzerland
Rothen-Rutishauser, Barbara Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland - Respiratory Medicine, Bern University Hospital, Switzerland
Petri-Fink, Alke Adolphe Merkle Institute, University of Fribourg, Marly, Switzerland - Chemistry Department, University of Fribourg, Switzerland

21.01.2012

Published in:

IEEE Transactions on Magnetics. - 2012, vol. 49, no. 1, p. 166-171

English Nanocarriers, including liposomes, offer great opportunities for targeted and controlled therapy. The development in this field has led to a large panel of drug delivery systems, which can be classified into 3 different nanovector generations. However, the success of such smart materials requires the control of a large variety of properties and parameters. Unfortunately, characterization at the nanoscale is often cumbersome and many methods are still being developed. Liposomes have been characterized by cryogenic electron microscopy (CryoTEM) for quite some time, also in combination with nanoparticles, in particular with superparamagnetic iron oxide nanoparticles (SPIONs) incorporated inside the liposomal membrane. CryoTEM, unlike classical TEM, maintains the native state of the liposomes. The quick freezing of the sample immobilizes particles and liposomes exactly at their position in the suspension. Therefore, localization information can be extracted from the images. However, data must be treated extremely carefully keeping in mind that 2-D projections of a 3-D object are observed. In this paper, we discuss the analysis of cryoTEM images of liposome-particle hybrids, including the estimation of the contrast transfer function (CTF) and electron dose, as well as the correct positioning of the sample holder and tomography for accurate localization.

Faculty

Faculté des sciences et de médecine

Department

Département de Chimie

Language