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Arabidopsis TWISTED DWARF1 functionally interacts with auxin exporter ABCB1 on the root plasma membrane

  • Wang, Bangjun Department of Biology-Plant Biology, University of Fribourg, Switzerland - University of Zurich, Institute of Plant Biology, Switzerland - Department of Plant and Environmental Sciences, University of Copenhagen, Denmark
  • Bailly, Aurélien Department of Biology-Plant Biology, University of Fribourg, Switzerland - University of Zurich, Institute of Plant Biology, Switzerland
  • Zwiewka, Marta Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium
  • Henrichs, Sina University of Zurich, Institute of Plant Biology, Switzerland
  • Azzarello, Elisa Department of Horticulture, University of Firenze, Italy
  • Mancuso, Stefano Department of Horticulture, University of Firenze, Italy
  • Maeshima, Masayoshi Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Japan
  • Friml, Jiří Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium - Department of Functional Genomics and Proteomics, Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic - Institute of Science and Technology Austria (IST Austria), Klosterneuburg, Austria
  • Schulz, Alexander Department of Plant and Environmental Sciences, University of Copenhagen, Denmark
  • Geisler, Markus Department of Biology-Plant Biology, University of Fribourg, Switzerland
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    15.01.2013
Published in:
  • The Plant Cell. - 2013, vol. 25, no. 1, p. 202-214
English Plant architecture is influenced by the polar, cell-to-cell transport of auxin that is primarily provided and regulated by plasma membrane efflux catalysts of the PIN-FORMED and B family of ABC transporter (ABCB) classes. The latter were shown to require the functionality of the FK506 binding protein42 TWISTED DWARF1 (TWD1), although underlying mechanisms are unclear. By genetic manipulation of TWD1 expression, we show here that TWD1 affects shootward root auxin reflux and, thus, downstream developmental traits, such as epidermal twisting and gravitropism of the root. Using immunological assays, we demonstrate a predominant lateral, mainly outward-facing, plasma membrane location for TWD1 in the root epidermis characterized by the lateral marker ABC transporter G36/PLEIOTROPIC DRUG-RESISTANCE8/PENETRATION3. At these epidermal plasma membrane domains, TWD1 colocalizes with nonpolar ABCB1. In planta bioluminescence resonance energy transfer analysis was used to verify specific ABC transporter B1 (ABCB1)–TWD1 interaction. Our data support a model in which TWD1 promotes lateral ABCB-mediated auxin efflux via protein–protein interaction at the plasma membrane, minimizing reflux from the root apoplast into the cytoplasm.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302830
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