Journal article

Analysis of cell identity, morphology, apoptosis and mitotic activity in a primary neural cell culture system in Drosophila

  • Moraru, Manuela M Cell and Developmental Biology Unit, Department of Biology, University of Fribourg, Switzerland
  • Egger, Boris Cell and Developmental Biology Unit, Department of Biology, University of Fribourg, Switzerland
  • Bao, Diarra B. Cell and Developmental Biology Unit, Department of Biology, University of Fribourg, Switzerland
  • Sprecher, Simon G. Cell and Developmental Biology Unit, Department of Biology, University of Fribourg, Switzerland
Show more…
    20.06.2012
Published in:
  • Neural Development. - 2012, vol. 7, p. 14
English In Drosophila, most neurogenetic research is carried out in vivo. Mammalian research demonstrates that primary cell culture techniques provide a powerful model to address cell autonomous and non-autonomous processes outside their endogenous environment. We developed a cell culture system in Drosophila using wildtype and genetically manipulated primary neural tissue for long-term observations. We assessed the molecular identity of distinct neural cell types by immunolabeling and genetically expressed fluorescent cell markers. We monitored mitotic activity of cell cultures derived from wildtype and tumorous larval brains. Our system provides a powerful approach to unveil developmental processes in the nervous system and to complement studies in vivo.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302722
Statistics

Document views: 56 File downloads:
  • spr_aci.pdf: 99