ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Ding, Zhaojun; Wang, Bangjun; Moreno, Ignacio; Dupláková, Nikoleta; Simon, Sibu; Carraro, Nicola; Reemmer, Jesica; Pěnčík, Aleš; Chen, Xu; Tejos, Ricardo; Skůpa, Petr; Pollmann, Stephan; Mravec, Jozef; Petrášek, Jan; Zažímalová, Eva; Honys, David; Rolčík, Jakub; Murphy, Angus; Orellana, Ariel; Geisler, Markus; Friml, Jiří

doi:10.1038/ncomms1941

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ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Ding, Zhaojun Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium - The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Shanda Nanlu 27, Jinan, China
Wang, Bangjun Department of Biology, Plant Biology, University of Fribourg, Switzerland
Moreno, Ignacio Center for Genome Regulation, Center of Plant Biotechnology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
Dupláková, Nikoleta Institute of Experimental Botany, Praha, Czech Republic
Simon, Sibu Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
Carraro, Nicola Department of Horticulture, Purdue University, West Lafayette, USA
Reemmer, Jesica Department of Horticulture, Purdue University, West Lafayette, USA
Pěnčík, Aleš Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
Chen, Xu Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
Tejos, Ricardo Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
Skůpa, Petr Institute of Experimental Botany, Praha, Czech Republic
Pollmann, Stephan Ruhr-Universität Bochum, Lehrstuhl für Pflanzenphysiologie, Germany
Mravec, Jozef Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
Petrášek, Jan Institute of Experimental Botany, Praha, Czech Republic
Zažímalová, Eva Institute of Experimental Botany, Praha, Czech Republic
Honys, David Institute of Experimental Botany, Praha, Czech Republic
Rolčík, Jakub Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
Murphy, Angus Department of Horticulture, Purdue University, West Lafayette, USA
Orellana, Ariel Center for Genome Regulation, Center of Plant Biotechnology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
Geisler, Markus Department of Biology, Plant Biology, University of Fribourg, Switzerland
Friml, Jiří Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium - Department of Functional Genomics and Proteomics, Faculty of Science and CEITEC, Masaryk University, Brno, Czech Republic

03.04.2012

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Nature Communications. - 2012, vol. 3, no. 1, p. 941

English Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language