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ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

  • Ding, Zhaojun Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium - The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Shanda Nanlu 27, Jinan, China
  • Wang, Bangjun Department of Biology, Plant Biology, University of Fribourg, Switzerland
  • Moreno, Ignacio Center for Genome Regulation, Center of Plant Biotechnology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
  • Dupláková, Nikoleta Institute of Experimental Botany, Praha, Czech Republic
  • Simon, Sibu Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
  • Carraro, Nicola Department of Horticulture, Purdue University, West Lafayette, USA
  • Reemmer, Jesica Department of Horticulture, Purdue University, West Lafayette, USA
  • Pěnčík, Aleš Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
  • Chen, Xu Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
  • Tejos, Ricardo Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
  • Skůpa, Petr Institute of Experimental Botany, Praha, Czech Republic
  • Pollmann, Stephan Ruhr-Universität Bochum, Lehrstuhl für Pflanzenphysiologie, Germany
  • Mravec, Jozef Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium
  • Petrášek, Jan Institute of Experimental Botany, Praha, Czech Republic
  • Zažímalová, Eva Institute of Experimental Botany, Praha, Czech Republic
  • Honys, David Institute of Experimental Botany, Praha, Czech Republic
  • Rolčík, Jakub Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, Czech Republic
  • Murphy, Angus Department of Horticulture, Purdue University, West Lafayette, USA
  • Orellana, Ariel Center for Genome Regulation, Center of Plant Biotechnology, Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile
  • Geisler, Markus Department of Biology, Plant Biology, University of Fribourg, Switzerland
  • Friml, Jiří Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Genetics, Ghent University, Belgium - Department of Functional Genomics and Proteomics, Faculty of Science and CEITEC, Masaryk University, Brno, Czech Republic
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    03.04.2012
Published in:
  • Nature Communications. - 2012, vol. 3, no. 1, p. 941
English Auxin is a key coordinative signal required for many aspects of plant development and its levels are controlled by auxin metabolism and intercellular auxin transport. Here we find that a member of PIN auxin transporter family, PIN8 is expressed in male gametophyte of Arabidopsis thaliana and has a crucial role in pollen development and functionality. Ectopic expression in sporophytic tissues establishes a role of PIN8 in regulating auxin homoeostasis and metabolism. PIN8 co-localizes with PIN5 to the endoplasmic reticulum (ER) where it acts as an auxin transporter. Genetic analyses reveal an antagonistic action of PIN5 and PIN8 in the regulation of intracellular auxin homoeostasis and gametophyte as well as sporophyte development. Our results reveal a role of the auxin transport in male gametophyte development in which the distinct actions of ER-localized PIN transporters regulate cellular auxin homoeostasis and maintain the auxin levels optimal for pollen development and pollen tube growth.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302626
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