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Structural basis for the molecular evolution of SRP-GTPase activation by protein

  • Bange, Gert Heidelberg University Biochemistry Center, Germany
  • Kümmerer, Nico Heidelberg University Biochemistry Center, Germany
  • Grudnik, Przemyslaw Heidelberg University Biochemistry Center, Germany
  • Lindner, Robert Heidelberg University Biochemistry Center, Germany
  • Petzold, Georg Heidelberg University Biochemistry Center, Germany - Research Institute of Molecular Pathology, Vienna, Austria
  • Kressler, Dieter Heidelberg University Biochemistry Center, Germany - Unit of Biochemistry, University of Fribourg, Switzerland
  • Hurt, Ed Heidelberg University Biochemistry Center, Germany
  • Wild, Klemens Heidelberg University Biochemistry Center, Germany
  • Sinning, Irmgard Heidelberg University Biochemistry Center, Germany
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    06.11.2011
Published in:
  • Nature Structural & Molecular Biology. - 2011, vol. 18, p. 1376–1380
English Small G proteins have key roles in signal transduction pathways. They are switched from the signaling 'on' to the non-signaling 'off' state when GTPase-activating proteins (GAPs) provide a catalytic residue. The ancient signal recognition particle (SRP)-type GTPases form GTP-dependent homo- and heterodimers and deviate from the canonical switch paradigm in that no GAPs have been identified. Here we show that the YlxH protein activates the SRP-GTPase FlhF. The crystal structure of the Bacillus subtilis FlhF–effector complex revealed that the effector does not contribute a catalytic residue but positions the catalytic machinery already present in SRP-GTPases. We provide a general concept that might also apply to the RNA-driven activation of the universally conserved, co-translational protein-targeting machinery comprising the SRP-GTPases Ffh and FtsY. Our study exemplifies the evolutionary transition from RNA- to protein-driven activation in SRP-GTPases and suggests that the current view on SRP-mediated protein targeting is incomplete.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302350
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