A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants

Barbez, Elke; Kubeš, Martin; Rolčík, Jakub; Béziat, Chloé; Pěnčík, Aleš; Wang, Bangjun; Rosquete, Michel Ruiz; Zhu, Jinsheng; Dobrev, Petre I.; Lee, Yuree; Zažímalovà, Eva; Petrášek, Jan; Geisler, Markus; Friml, Jiří; Kleine-Vehn, Jürgen

doi:10.1038/nature11001

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A novel putative auxin carrier family regulates intracellular auxin homeostasis in plants

Barbez, Elke Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium - Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Siences Vienna, Austria
Kubeš, Martin Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, Praha, Czech Republic
Rolčík, Jakub
Béziat, Chloé Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium - Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Siences Vienna, Austria
Pěnčík, Aleš Centre of the Region Hana´ for Biotechnological and Agricultural Research, Department of Growth Regulators, Faculty of Science, Olomouc, Czech Republic
Wang, Bangjun Department of Biology - Plant Biology, University of Fribourg, Switzerland
Rosquete, Michel Ruiz Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium - Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Siences Vienna, Austria
Zhu, Jinsheng Department of Biology - Plant Biology, University of Fribourg, Switzerland
Dobrev, Petre I. Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, Praha, Czech Republic
Lee, Yuree Department of Plant Molecular Biology, University of Lausanne, Switzerland
Zažímalovà, Eva Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, Praha, Czech Republic
Petrášek, Jan Institute of Experimental Botany, The Academy of Sciences of the Czech Republic, Praha, Czech Republic
Geisler, Markus Department of Biology - Plant Biology, University of Fribourg, Switzerland
Friml, Jiří Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium
Kleine-Vehn, Jürgen Department of Plant Systems Biology, VIB and Department of Plant Biotechnology and Bioinformatics, Ghent University, Belgium - Department of Applied Genetics and Cell Biology, University of Natural Resources and Life Siences Vienna, Austria

15.04.2012

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Nature. - 2012, vol. 485, no. 7396, p. 119-122

English The phytohormone auxin acts as a prominent signal, providing, by its local accumulation or depletion in selected cells, a spatial and temporal reference for changes in the developmental program. The distribution of auxin depends on both auxin metabolism (biosynthesis, conjugation and degradation) and cellular auxin transport. We identified in silico a novel putative auxin transport facilitator family, called PIN-LIKES (PILS). Here we illustrate that PILS proteins are required for auxin-dependent regulation of plant growth by determining the cellular sensitivity to auxin. PILS proteins regulate intracellular auxin accumulation at the endoplasmic reticulum and thus auxin availability for nuclear auxin signalling. PILS activity affects the level of endogenous auxin indole-3-acetic acid (IAA), presumably via intracellular accumulation and metabolism. Our findings reveal that the transport machinery to compartmentalize auxin within the cell is of an unexpected molecular complexity and demonstrate this compartmentalization to be functionally important for a number of developmental processes.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language