Hepatic mitochondrial energetics during catch-Up fat with high-Fat diets rich in lard or safflower oil
- Crescenzo, Raffaella Department of Structural and Functional Biology, University of Naples, Italy
- Bianco, Francesca Department of Structural and Functional Biology, University of Naples, Italy
- Falcone, Italia Department of Structural and Functional Biology, University of Naples, Italy
- Tsalouhidou, Sofia Department of Physical Education and Sport Science, University of Thessaloniki, Greece
- Yepuri, Gayathri Department of Medicine, Division of Physiology, University of Fribourg, Switzerland
- Mougios, Vassilis Department of Physical Education and Sport Science, University of Thessaloniki, Greece
- Dulloo, Abdul G. Department of Medicine, Division of Physiology, University of Fribourg, Switzerland
- Liverini, Giovanna Department of Structural and Functional Biology, University of Naples, Italy
- Iossa, Susanna Department of Structural and Functional Biology, University of Naples, Italy
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30.06.2011
Published in:
- Obesity. - 2012, vol. 20, no. 9, p. 1763-1772
English
We have investigated whether altered hepatic mitochondrial energetics could explain the differential effects of high-fat diets with low or high ω6 polyunsaturated fatty acid content (lard vs. safflower oil) on the efficiency of body fat recovery (catch-up fat) during refeeding after caloric restriction. After 2 weeks of caloric restriction, rats were isocalorically refed with a low-fat diet (LF) or high-fat diets made from either lard or safflower oil for 1 week, and energy balance and body composition changes were assessed. Hepatic mitochondrial energetics were determined from measurements of liver mitochondrial mass, respiratory capacities, and proton leak. Compared to rats refed the LF, the groups refed high-fat diets showed lower energy expenditure and increased efficiency of fat gain; these differences were less marked with high-safflower oil than with high-lard diet. The increase in efficiency of catch-up fat by the high-fat diets could not be attributed to differences in liver mitochondrial activity. By contrast, the lower fat gain with high-safflower oil than with high-lard diet is accompanied by higher mitochondrial proton leak and increased proportion of arachidonic acid in mitochondrial membranes. In conclusion, the higher efficiency for catch-up fat on high-lard diet than on LF cannot be explained by altered hepatic mitochondrial energetics. By contrast, the ability of the high-safflower oil diet to produce a less pronounced increase in the efficiency of catch-up fat may partly reside in increased incorporation of arachidonic acid in hepatic mitochondrial membranes, leading to enhanced proton leak and mitochondrial uncoupling.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Médecine
- Language
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- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
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- RERO DOC 28160
- DOI 10.1038/oby.2011.167
- Persistent URL
- https://folia.unifr.ch/unifr/documents/302163
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