Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection
- Zanetti, Simone Department of Biology, University of Fribourg, Switzerland
- Meola, Marco Department of Biology, University of Fribourg, Switzerland
- Bochud, Arlette Department of Biology, University of Fribourg, Switzerland
- Puoti, Alessandro Department of Biology, University of Fribourg, Switzerland
-
12.04.2011
Published in:
- Developmental Biology. - 2011, vol. 354, no. 2, p. 232-241
English
In Caenorhabditis elegans, germ cells develop as spermatids in the larva and as oocytes in the adult. Such fundamentally different gametes are produced through a fine-tuned balance between feminizing and masculinizing genes. For example, the switch to oogenesis requires repression of the fem-3 mRNA through the mog genes. Here we report on the cloning and characterization of the sex determination gene mog-2. MOG-2 is the worm homolog of spliceosomal protein U2A′. We found that MOG-2 is expressed in most nuclei of somatic and germ cells. In addition to its role in sex determination, mog-2 is required for meiosis. Moreover, MOG-2 binds to U2B″/RNP-3 in the absence of RNA. We also show that MOG-2 associates with the U2 snRNA in the absence of RNP-3. Therefore, we propose that MOG-2 is a bona fide component of the U2 snRNP. Albeit not being required for general pre-mRNA splicing, MOG-2 increases the splicing efficiency to a cryptic splice site that is located at the 5′ end of the exon.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
-
- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
-
- RERO DOC 24865
- DOI 10.1016/j.ydbio.2011.04.001
- Persistent URL
- https://folia.unifr.ch/unifr/documents/302155
Statistics
Document views: 48
File downloads:
- puo_rup.pdf: 89