Journal article

Role of the C. elegans U2 snRNP protein MOG-2 in sex determination, meiosis, and splice site selection

  • Zanetti, Simone Department of Biology, University of Fribourg, Switzerland
  • Meola, Marco Department of Biology, University of Fribourg, Switzerland
  • Bochud, Arlette Department of Biology, University of Fribourg, Switzerland
  • Puoti, Alessandro Department of Biology, University of Fribourg, Switzerland
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    12.04.2011
Published in:
  • Developmental Biology. - 2011, vol. 354, no. 2, p. 232-241
English In Caenorhabditis elegans, germ cells develop as spermatids in the larva and as oocytes in the adult. Such fundamentally different gametes are produced through a fine-tuned balance between feminizing and masculinizing genes. For example, the switch to oogenesis requires repression of the fem-3 mRNA through the mog genes. Here we report on the cloning and characterization of the sex determination gene mog-2. MOG-2 is the worm homolog of spliceosomal protein U2A′. We found that MOG-2 is expressed in most nuclei of somatic and germ cells. In addition to its role in sex determination, mog-2 is required for meiosis. Moreover, MOG-2 binds to U2B″/RNP-3 in the absence of RNA. We also show that MOG-2 associates with the U2 snRNA in the absence of RNP-3. Therefore, we propose that MOG-2 is a bona fide component of the U2 snRNP. Albeit not being required for general pre-mRNA splicing, MOG-2 increases the splicing efficiency to a cryptic splice site that is located at the 5′ end of the exon.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/302155
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