Coexistence of copy number increases of c-Myc, ZNF217, CCND1, ErbB1 and ErbB2 in ovarian cancers

Dimova, Ivanka; Raicheva, Sashka; Dimitrov, Rumen; Doganov, Nikolai; Toncheva, Draga

doi:10.1159/000219368

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Journal article

Coexistence of copy number increases of c-Myc, ZNF217, CCND1, ErbB1 and ErbB2 in ovarian cancers

Dimova, Ivanka Department of Medical Genetics, Medical University Sofia, Bulgaria - Institute of Anatomy, University of Fribourg, Switzerland
Raicheva, Sashka Laboratory of Gynecopathology, University Hospital of Obstetrics and Gynecology, Sofia, Bulgaria
Dimitrov, Rumen Second Gynecologic Clinic, University Hospital of Obstetrics and Gynecology, Sofia, Bulgaria
Doganov, Nikolai Second Gynecologic Clinic, University Hospital of Obstetrics and Gynecology, Sofia, Bulgaria
Toncheva, Draga Department of Medical Genetics, Medical University Sofia, Bulgaria

20.06.2009

Published in:

Onkologie. - 2009, vol. 32, no. 7, p. 405-410

Fluorescence in situ hybridization

English Background: We selected 5 oncogenes with well-established roles in carcinogenesis – CCND1, ErbB1, ErbB2, c-mycand ZNF217– to investigate the coexistence of their copy imbalances in relation to the clinico-pathological characteristics of ovarian tumors. Materials and Methods: Fluorescence in situ hybridization for the 5 genes was applied to a preexisting tissue microarray. 38 ovarian tumors were successfully analyzed for copy number changes of the 5 genes. Results: At least one of these oncogenes was gained/amplified in 27 out of 38 tumors (71.1). We report the highest frequency of c-mycgenetic gain/amplification since it affected 42.1 of the ovarian tumors. We observed sequential involvement of copy number alterations of the other genes in the presence of c-mycdisruption. The incidence of copy number changes of the 5 oncogenes – both single and combinatorial – was higher in high-grade tumors. All double aberrations in the serous group comprised c-mycand ZNF217copy number increases. Conclusions: Our results revealed a combination between copy number increases of c-mycand ZNF217, associated with serous histology. The data from this combined analysis of the 5 oncogenes could be used as a basis in considering the combined approach in molecular-based therapy of ovarian cancer.

Faculty

Faculté des sciences et de médecine

Department

Département de Médecine

Language