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Multiple promoters contribute to swarming and the coordination of transcription with flagellar assembly in Salmonella

  • Wozniak, Christopher E. Department of Microbiology, University of Washington, Seattle, USA - Department of Biology, University of Utah, Salt Lake City, USA
  • Chevance, Fabienne F. V. Department of Biology, University of Utah, Salt Lake City, USA - Department of Medicine, University of Fribourg, Switzerland
  • Hughes, Kelly T. Department of Biology, University of Utah, Salt Lake City, USA - Department of Medicine, University of Fribourg, Switzerland
    16.07.2010
Published in:
  • Journal of Bacteriology. - 2010, vol. 192, no. 18, p. 4752-4762
English In Salmonella, there are three classes of promoters in the flagellar transcriptional hierarchy. This organization allows genes needed earlier in the construction of flagella to be transcribed before genes needed later. Four operons (fliAZY, flgMN, fliDST, and flgKL) are expressed from both class 2 and class 3 promoters. To investigate the purpose for expressing genes from multiple flagellar promoters, mutants were constructed for each operon that were defective in either class 2 transcription or class 3 transcription. The mutants were checked for defects in swimming through liquids, swarming over surfaces, and transcriptional regulation. The expression of the hook-associated proteins (FlgK, FlgL, and FliD) from class 3 promoters was found to be important for swarming motility. Both flgMN promoters were involved in coordinating class 3 transcription with the stage of assembly of the hook-basal body. Finally, the fliAZY class 3 promoter lowered class 3 transcription in stationary phase. These results indicate that the multiple flagellar promoters respond to specific environmental conditions and help coordinate transcription with flagellar assembly.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biological sciences
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/301843
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