Journal article

Biomedical nanoparticles modulate specific CD4+ T cell stimulation by inhibition of antigen processing in dendritic cells

  • Blank, Fabian Department of Clinical Research, Division of Pulmonology, University Hospital, Bern, Switzerland
  • Gerber, Peter Department of Clinical Research, Division of Pulmonology, University Hospital, Bern, Switzerland
  • Rothen-Rutishauser, Barbara Institute of Anatomy, Division of Histology, University of Bern, Switzerland
  • Sakulkhu, Usawadee Powder Technology Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland
  • Salaklang, Jatuporn Powder Technology Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Peyer, Karin De Department of Clinical Research, Division of Pulmonology, University Hospital, Bern, Switzerland
  • Gehr, Peter Institute of Anatomy, Division of Histology, University of Bern, Switzerland
  • Nicod, Laurent P. Division of Pulmonology, University Hospital, Lausanne, Switzerland
  • Hofmann, Heinrich Powder Technology Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland
  • Geiser, Thomas Department of Clinical Research, Division of Pulmonology, University Hospital, Bern, Switzerland
  • Petri-Fink, Alke Powder Technology Laboratory, École Polytechnique Fédérale de Lausanne, Switzerland - Department of Chemistry, University of Fribourg, Switzerland
  • Garnier, Christophe von Department of Clinical Research, Division of Pulmonology, University Hospital, Bern, Switzerland
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    2011
Published in:
  • Nanotoxicology. - 2011, vol. 5, no. 4, p. 606-621
English Understanding how nanoparticles may affect immune responses is an essential prerequisite to developing novel clinical applications. To investigate nanoparticle-dependent outcomes on immune responses, dendritic cells (DCs) were treated with model biomedical poly(vinylalcohol)-coated super-paramagnetic iron oxide nanoparticles (PVA-SPIONs). PVA-SPIONs uptake by human monocyte-derived DCs (MDDCs) was analyzed by flow cytometry (FACS) and advanced imaging techniques. Viability, activation, function, and stimulatory capacity of MDDCs were assessed by FACS and an in vitro CD4⁺ T cell assay. PVA-SPION uptake was dose-dependent, decreased by lipopolysaccharide (LPS)-induced MDDC maturation at higher particle concentrations, and was inhibited by cytochalasin D pre-treatment. PVA-SPIONs did not alter surface marker expression (CD80, CD83, CD86, myeloid/plasmacytoid DC markers) or antigen-uptake, but decreased the capacity of MDDCs to process antigen, stimulate CD4⁺ T cells, and induce cytokines. The decreased antigen processing and CD4⁺ T cell stimulation capability of MDDCs following PVA-SPION treatment suggests that MDDCs may revert to a more functionally immature state following particle exposure.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/301827
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