Different Mi-2 Complexes for Various Developmental Functions in Caenorhabditis elegans
- Passannante, Myriam Department of Biology, University of Fribourg, Switzerland
- Marti, Claude-Olivier Department of Biology, University of Fribourg, Switzerland
- Pfefferli, Catherine Department of Biology, University of Fribourg, Switzerland
- Moroni, Paolo S. Department of Biology, University of Fribourg, Switzerland
- Kaeser-Pebernard, Stéphanie Department of Biology, University of Fribourg, Switzerland
- Puoti, Alessandro Department of Biology, University of Fribourg, Switzerland
- Hunziker, Peter Functional Genomics Center Zürich, University/ETH Zurich, Switzerland
- Wicky, Chantal Department of Biology, University of Fribourg, Switzerland
- Müller, Fritz Department of Biology, University of Fribourg, Switzerland
-
27.10.2010
Published in:
- PLoS ONE. - 2010, vol. 5, no. 10, p. e13681
English
Biochemical purifications from mammalian cells and Xenopus oocytes revealed that vertebrate Mi-2 proteins reside in multisubunit NuRD (Nucleosome Remodeling and Deacetylase) complexes. Since all NuRD subunits are highly conserved in the genomes of C. elegans and Drosophila, it was suggested that NuRD complexes also exist in invertebrates. Recently, a novel dMec complex, composed of dMi-2 and dMEP-1 was identified in Drosophila. The genome of C. elegans encodes two highly homologous Mi-2 orthologues, LET-418 and CHD-3. Here we demonstrate that these proteins define at least three different protein complexes, two distinct NuRD complexes and one MEC complex. The two canonical NuRD complexes share the same core subunits HDA-1/HDAC, LIN-53/RbAp and LIN-40/MTA, but differ in their Mi-2 orthologues LET-418 or CHD-3. LET-418 but not CHD-3, interacts with the Krüppel-like protein MEP-1 in a distinct complex, the MEC complex. Based on microarrays analyses, we propose that MEC constitutes an important LET-418 containing regulatory complex during C. elegans embryonic and early larval development. It is required for the repression of germline potential in somatic cells and acts when blastomeres are still dividing and differentiating. The two NuRD complexes may not be important for the early development, but may act later during postembryonic development. Altogether, our data suggest a considerable complexity in the composition, the developmental function and the tissue-specificity of the different C. elegans Mi-2 complexes.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
-
- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
-
- RERO DOC 21060
- DOI 10.1371/journal.pone.0013681
- Persistent URL
- https://folia.unifr.ch/unifr/documents/301760
Statistics
Document views: 53
File downloads:
- mue_dmc.pdf: 79