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Scattering information obtained by optical microscopy: Differential dynamic microscopy and beyond

  • Giavazzi, Fabio Dipartimento di Chimica, Biochimica a Biotecnologie per la Medicina, Università degli Studi di Milano, Milan, Italy
  • Brogioli, Doriano Dipartimento di Medicina Sperimentale, Università degli Studi di Milano–Bicocca, Milano, Italy
  • Trappe, Véronique Department of Physics, University of Fribourg, Switzerland
  • Bellini, Tommaso Dipartimento di Chimica, Biochimica a Biotecnologie per la Medicina, Università degli Studi di Milano, Milan, Italy
  • Cerbino, Roberto Dipartimento di Chimica, Biochimica a Biotecnologie per la Medicina, Università degli Studi di Milano, Milan, Italy
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    21.09.2009
Published in:
  • Physical Review E. - 2009, vol. 80, no. 3, p. 031403
Colloids
Fluorescence
Fourier transform optics
Light scattering
Optical microscopy
English We describe the use of a bright-field microscope for dynamic light scattering experiments on weakly scattering samples. The method is based on collecting a time sequence of microscope images and analyzing them in the Fourier space to extract the characteristic time constants as a function of the scattering wave vector. We derive a theoretical model for microscope imaging that accounts for (a) the three-dimensional nature of the sample, (b) the arbitrary coherence properties of the light source, and (c) the effect of the finite numerical aperture of the microscope objective. The model is tested successfully against experiments performed on a colloidal dispersion of small spheres in water, by means of the recently introduced differential dynamic microscopy technique [R. Cerbino and V. Trappe, Phys. Rev. Lett. 100, 188102 (2008)]. Finally, we extend our model to the class of microscopy techniques that can be described by a linear space-invariant imaging of the density of the scattering centers, which includes, for example, dynamic fluorescence microscopy.
Faculty
Faculté des sciences et de médecine
Department
Département de Physique
Language
  • English
Classification
Physics
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/301456
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