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Improved in vivo two-photon imaging after blood replacement by perfluorocarbon

  • Haiss, F. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
  • Jolivet, R. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
  • Wyss, M. T. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
  • Reichold, J. Institute of Fluid Dynamics, ETH Zurich, Switzerland
  • Ben Braham, Nasser Department of Physics and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Scheffold, Frank Department of Physics and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
  • Krafft, M. P. Université de Strasbourg, Institut Charles Sadron (CNRS France
  • Weber, B. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
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    24.04.2009
Published in:
  • The Journal of Physiology. - 2009, vol. 587, p. 3153-3158
English Two-photon microscopy is a powerful method in biomedical research that allows functional and anatomical imaging at a subcellular resolution in vivo. The technique is seriously hampered by absorption and scattering of light by blood, which prevents imaging through large vessels. Here, we demonstrate in the rat cerebral cortex that blood replacement by perfluorocarbon emulsion, a compound also used in human critical care medicine, yields superior image quality, while preserving neuronal integrity. Shadows of large superficial vessels disappear completely and cells can be imaged underneath them. For the first time, it is possible to image complete populations of neurons and astrocytes in the upper layers of neocortex in vivo.
Faculty
Faculté des sciences
Department
Physique
Language
  • English
Classification
Physics
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/301439
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