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O-linked β-N-acetylglucosamine during hyperglycemia exerts both anti-inflammatory and pro-oxidative properties in the endothelial system

  • Rajapakse, Angana Gupta Vascular Biology, Division of Physiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
  • Ming, Xiu-Fen Vascular Biology, Division of Physiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
  • Carvas, João Miguel Vascular Biology, Division of Physiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
  • Yang, Zhihong Vascular Biology, Division of Physiology, Department of Medicine, Faculty of Science, University of Fribourg, Switzerland
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    2009
Published in:
  • Oxidative Medicine & Cellular Longevity. - 2009, vol. 2, no. 3, p. 172-175
Adhesion molecules
Endothelial cells
Glucosamine
Inflammation
ROS
English Elevated cellular levels of protein O-linked β-N-acetylglucosamine (O-GlcNAc) through hexosamine biosynthesis pathway (HBP) are suggested to contribute to cardiovascular adverse effects under chronic hyperglycemic condition associated with oxidative stress and inflammation. Conversely, enhancing O- GlcNAc levels have also been demonstrated being protective against myocardial ischemia/reperfusion injury. We recently demonstrated that hyperglycemia increases oxidative stress and HBP flux in endothelial cells and enhances endothelial expression of vascular adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) in response to tumor necrosis factor- α (TNF-α) through oxidative stress rather than HBP pathway. Here we present further complementary data showing that enhancing O-GlcNAc levels by glucosamine does not mimic hyperglycemia’s effect on TNF-α−induced endothelial VCAM-1 and ICAM-1 expression. Glucosamine however inhibits ICAM-1 (not VCAM-1) expression and induces superoxide generation in the cells. The results further suggest that increased O-GlcNAc levels do not mediate the enhancing effect of hyperglycemia on the endothelial inflammatory responses to TNF-α. In contrast, it exerts certain anti-inflammatory effects accompanied by pro-oxidative properties. Further work should delineate the exact role of HPB pathway in different aspects of cardiovascular functions, especially those of diabetic cardiovascular complications.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/301432
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