Photoinitiated coupling of unmodified monosaccharides to iron oxide nanoparticles for sensing proteins and bacteria
- Liu, Li-Hong Department of Chemistry, Portland State University, USA
- Dietsch, Hervé Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
- Schurtenberger, Peter Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
- Yan, Mingdi Department of Chemistry, Portland State University, USA
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17.06.2009
Published in:
- Bioconjugate Chemistry. - 2009, vol. 20, no. 7, p. 1349–1355
English
We report a versatile approach for the immobilization of unmodified monosaccharides onto iron oxide nanoparticles. Covalent coupling of the carbohydrate onto iron oxide nanoparticle surfaces was accomplished by the CH insertion reaction of photochemically activated phosphate-functionalized perfluorophenylazides (PFPAs), and the resulting glyconanoparticles were characterized by IR, TGA, and TEM. The surface-bound d-mannose showed the recognition ability toward Concanavalin A and Escherichia coli strain ORN178 that possesses mannose-specific receptor sites. Owing to the simplicity and versatility of the technique, together with the magnetic property of iron oxide nanoparticles, the methodology developed in this study serves as a general approach for the preparation of magnetic glyconanoparticles to be used in clinical diagnosis, sensing, and decontamination.
- Faculty
- Faculté des sciences et de médecine
- Department
- AMI - Soft Nanoscience
- Language
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- English
- Classification
- Biotechnology
- License
- License undefined
- Identifiers
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- RERO DOC 13249
- DOI 10.1021/bc900110x
- Persistent URL
- https://folia.unifr.ch/unifr/documents/301347
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