Photoinitiated coupling of unmodified monosaccharides to iron oxide nanoparticles for sensing proteins and bacteria

Liu, Li-Hong; Dietsch, Hervé; Schurtenberger, Peter; Yan, Mingdi

doi:10.1021/bc900110x

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Photoinitiated coupling of unmodified monosaccharides to iron oxide nanoparticles for sensing proteins and bacteria

Liu, Li-Hong Department of Chemistry, Portland State University, USA
Dietsch, Hervé Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
Schurtenberger, Peter Adolphe Merkle Institute and Fribourg Center for Nanomaterials, University of Fribourg, Switzerland
Yan, Mingdi Department of Chemistry, Portland State University, USA

17.06.2009

Published in:

Bioconjugate Chemistry. - 2009, vol. 20, no. 7, p. 1349–1355

English We report a versatile approach for the immobilization of unmodified monosaccharides onto iron oxide nanoparticles. Covalent coupling of the carbohydrate onto iron oxide nanoparticle surfaces was accomplished by the CH insertion reaction of photochemically activated phosphate-functionalized perfluorophenylazides (PFPAs), and the resulting glyconanoparticles were characterized by IR, TGA, and TEM. The surface-bound d-mannose showed the recognition ability toward Concanavalin A and Escherichia coli strain ORN178 that possesses mannose-specific receptor sites. Owing to the simplicity and versatility of the technique, together with the magnetic property of iron oxide nanoparticles, the methodology developed in this study serves as a general approach for the preparation of magnetic glyconanoparticles to be used in clinical diagnosis, sensing, and decontamination.

Faculty

Faculté des sciences et de médecine

Department

AMI - Soft Nanoscience

Language