Journal article

+ 1 other files

Phosphorylation state defines discrete roles for monopolin in chromosome attachment and spindle elongation

  • Choi, Sung Hugh Department of Molecular Genetics and Microbiology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, USA
  • Péli-Gulli, Marie-Pierre EPFL SV ISREC UPSIM, Lausanne, Switzerland - Department of Medicine, Division of Biochemistry, University of Fribourg, Switzerland
  • Mcleod, Iain Department of Cell Biology, The Scripps Research Institute, La Jolla, USA
  • Sarkeshik, Ali Department of Cell Biology, The Scripps Research Institute, La Jolla, USA
  • Yates, John R. Department of Cell Biology, The Scripps Research Institute, La Jolla, USA
  • Simanis, Viesturs EPFL SV ISREC UPSIM, Lausanne, Switzerland
  • McCollum, Dannel Department of Molecular Genetics and Microbiology and Program in Cell Dynamics, University of Massachusetts Medical School, Worcester, USA
Show more…
    11.06.2009
Published in:
  • Current Biology. - 2009, vol. 19, no. 12, p. 985-995
English Background: it is unknown how oscillations in Cdk1 activity drive the dramatic changes in chromosome and spindle dynamics that occur at the metaphase/anaphase transition. Results: we show that the Schizosaccharomyces pombe monopolin complex has distinct functions in metaphase and anaphase that are determined by the phosphorylation state of its Mde4 subunit. When Cdk1 activity is high in metaphase, Mde4 is hyperphosphorylated on Cdk1 phosphorylation sites and localizes to kinetochores. A nonphosphorylatable mutant of Mde4 does not localize to kinetochores, appears prematurely on the metaphase spindle, and interferes with spindle dynamics and chromosome segregation, illustrating the importance of Cdk1 phosphorylation in regulating metaphase monopolin activity. When Cdk1 activity drops in anaphase, dephosphorylation of Mde4 triggers monopolin localization to the mitotic spindle, where it promotes spindle elongation and integrity, coupling the late mitotic loss of Cdk1 activity to anaphase spindle dynamics. Conclusions: together, these findings illustrate how the sequential phosphorylation and dephosphorylation of monopolin helps ensure the orderly execution of discrete steps in mitosis.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/301275
Other files

Statistics

Document views: 7 File downloads:
  • peligulli_psd.pdf: 1
  • peligulli_psd_sm.pdf: 1