Dynamic laser speckle imaging of cerebral blood flow
- Zakharov, Pavel Department of Physics, University of Fribourg, Switzerland -
- Völker, A. C. Department of Physics, University of Fribourg, Switzerland
- Wyss, M. T. Division of Nuclear Medicine, University Hospital Zurich, Switzerland - Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
- Haiss, F. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
- Calcinaghi, N. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
- Zunzunegui, C. Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
- Buck, Alfred Division of Nuclear Medicine, University Hospital Zurich, Switzerland
- Scheffold, Frank Department of Physics, University of Fribourg, Switzerland
- Weber, Bruno Institute of Pharmacology and Toxicology, University of Zurich, Switzerland
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2009
Published in:
- Optics Express. - 2009, vol. 17, no. 16, p. 13904-13917
English
Laser speckle imaging (LSI) based on the speckle contrast analysis is a simple and robust technique for imaging of heterogeneous dynamics. LSI finds frequent application for dynamical mapping of cerebral blood flow, as it features high spatial and temporal resolution. However, the quantitative interpretation of the acquired data is not straightforward for the common case of a speckle field formed by both by moving and localized scatterers such as blood cells and bone or tissue. Here we present a novel processing scheme, we call dynamic laser speckle imaging (dLSI), that can be used to correctly extract the temporal correlation parameters from the speckle contrast measured in the presence of a static or slow-evolving background. The static light contribution is derived from the measurements by cross-correlating sequential speckle images. In-vivo speckle imaging experiments performed in the rodent brain demonstrate that dLSI leads to improved results. The cerebral hemodynamic response observed through the thinned and intact skull are more pronounced in the dLSI images as compared to the standard speckle contrast analysis. The proposed method also yields benefits with respect to the quality of the speckle images by suppressing contributions of non-uniformly distributed specular reflections.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Physique
- Language
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- English
- Classification
- Physics
- License
- License undefined
- Identifiers
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- RERO DOC 13203
- DOI 10.1364/OE.17.013904
- Persistent URL
- https://folia.unifr.ch/unifr/documents/301265
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