Engrailed-2 regulates genes related to vesicle formation and transport in cerebellar Purkinje cells

Holst, Martin I.; Maercker, Christian; Pintea, Bogdan; Masseroli, Marco; Liebig, Christian; Jankowski, Jakob; Miething, Andreas; Martini, Julia; Schwaller, Beat; Oberdick, John; Schilling, Karl; Baader, Stephan L.

doi:10.1016/j.mcn.2008.04.010

Back

Journal article

Engrailed-2 regulates genes related to vesicle formation and transport in cerebellar Purkinje cells

Holst, Martin I. Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany
Maercker, Christian German Cancer Research Center (DKFZ), Genomics and Proteomics Core Facilities, Heidelberg, Germany - Mannheim University of Applied Sciences, Mannheim, Germany
Pintea, Bogdan Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany
Masseroli, Marco Department of Electronics and Information, Politecnico di Milano, Italy
Liebig, Christian Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany
Jankowski, Jakob Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany
Miething, Andreas Institute of Anatomy, Neuroanatomy, University of Bonn, Germany
Martini, Julia Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany
Schwaller, Beat Unit of Anatomy, Department of Medicine, University of Fribourg,Switzerland
Oberdick, John Department of Neuroscience, The Ohio State University College of Medicine, Columbus, Ohio, USA - Center for Molecular Neurobiology, The Ohio State University College of Medicine, Columbus, Ohio, USA
Schilling, Karl Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany
Baader, Stephan L. Institute of Anatomy, Anatomy and Cell Biology, University of Bonn, Germany - Institute of Anatomy I, University of Jena, Germany

02.05.2008

Published in:

Molecular and Cellular Neuroscience. - 2008, vol. 38, no. 4, p. 10.1016

English Engrailed transcription factors regulate survival, cell fate decisions and axon pathfinding in central neurons. En-2 can also attenuate Purkinje cell (PC) maturation. Here, we use array analysis to scrutinize gene expression in developing PCs overexpressing Engrailed-2 (L7En-2). The majority (70%) of regulated genes was found down-regulated in L7En-2 cerebella, consistent with the known repressive function of Engrailed-2. Differential gene expression, verified by in situ hybridization or Western blotting, was particularly evident during the first postnatal week, when L7En-2 PCs display conspicuous deficits in dendritogenesis. Functional classification revealed clusters of genes linked to vesicle formation and transport. Consistently, Golgi stacks located at the axonal pole of wild type PC somata were rarely detected in L7En-2 PCs. In addition, long continuous stretches of endoplasmic reticulum typically found around the axonal pole of wild type PCs were less frequently observed in transgenic cells. Engrailed-2 might therefore orchestrate PC survival and process formation as a regulator of subcellular organization.

Faculty

Faculté des sciences et de médecine

Department

Département de Médecine

Language