Enhanced tumour growth and impaired cellular antitumoural defence in hepatic colorectal carcinoma metastasis in rats after laparoscopy compared to open surgery

Inderbitzin, D. Th.; Marti, G. R.; Eichenberger, S.; Hoogewoud, Henri-Marcel; Krähenbühl, Lukas

doi:10.1245/s10434-007-9801-1

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Journal article

Enhanced tumour growth and impaired cellular antitumoural defence in hepatic colorectal carcinoma metastasis in rats after laparoscopy compared to open surgery

Inderbitzin, D. Th. Department of General Surgery, Hôpital Cantonal Fribourg, Switzerland
Marti, G. R. Department of Histology and Embryology, Faculty of Medicine, University of Fribourg, Switzerland
Eichenberger, S. Department of Histology and Embryology, Faculty of Medicine, University of Fribourg, Switzerland
Hoogewoud, Henri-Marcel Department of Radiology, Hôpital Cantonal Fribourg, Switzerland
Krähenbühl, Lukas Department of General Surgery, Hôpital Cantonal Fribourg, Switzerland - Department of Visceral Surgery, Lindenhof Spital, Berne, Switzerland

05.02.2008

Published in:

Annals of Surgical Oncology. - 2008, vol. 15, no. 4, p. p

English Background This study aims to assess postoperative hepatic growth of colorectal adenocarcinoma metastasis and peritumoural macrophage counts after laparoscopy in an experimental animal model. Methods Thirty male syngenic WAG/Rij rats were randomised into two surgical groups: laparoscopy (LS; n = 15) using CO₂ at 12 mmHg and laparotomy (LT; n = 15; negative control) during an operating time of 90 min. At 45 min after setup, CC531s colon adenocarcinoma cells were injected into two liver lobes. Postoperative tumour volumes were determined by abdominal magnetic resonance imaging (MRI) and computed three-dimensional volumetry. Peritumoural macrophages were counted by local stereology using a confocal laser-scanning fluorescence microscope. Results The median postoperative tumour volume was significantly higher after LS in both lobes (L): after 10, 15 and 20 days in L2 and L5: 24/12, 54/38, 275/62 mm³ and 0/0, 15/11, 55/24 mm³ (LS/LT). Significantly fewer peritumoural macrophages were found after LS at all postoperative time points (Mann–Whitney: p< 0.05). Conclusions Increased hepatic growth of colorectal adenocarcinoma metastasis and impaired cellular antitumoural defence after LS cast doubt on the use of LS in colorectal cancer and needs further clinical investigation.

Faculty

Faculté des sciences et de médecine

Department

Département de Médecine

Language