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Role for glutathione in the hyposensitivity of LPS-pretreated mice to LPS anorexia

  • Hernadfalvi, Noemi Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland
  • Langhans, Wolfgang Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland
  • Meyenburg, Claudia von Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland
  • Onteniente, Brigitte INSERM UMR421, Creteil, France
  • Richard, Denis Department of Anatomy and Physiology, Laval University, Quebec, Canada
  • Arsenijevic, Denis Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland - Division of Physiology, Department of Medicine, University of Fribourg, Switzerland
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    2007
Published in:
  • European Cytokine Network. - 2007, vol. 18, no. 2, p. 39-45
reactive oxygen species
cytokine
food intake
liver
brain
mitochondria
English To study the role of the redox state regulator glutathione (GSH) in bacterial lipopolysaccharide (LPS)-induced anorexia we measured GSH in liver, serum and brain in response to intraperitoneal (ip) lipopolysaccharide (LPS, 4μg/mouse) injection in LPS-naïve and LPS-pretreated (4 μg/mouse) mice. LPS reduced food intake in LPS-naïve mice and LPS pretreatment attenuated this effect. LPS reduced total reduced GSH at 24 hours after injection in LPS-naïve mice. On the other hand, LPS pretreatment caused an upregulation of GSH levels in brain and liver, and this was associated with a significant attenuation of LPS-induced anorexia. In addition, LPS pretreatment increased mitochondrial GSH levels in brain and liver. Pharmacological GSH depletion with diethylmaleate and L-buthionine sulfoximine in LPS-pretreated mice ablated the hyposensitivity to the anorexic effect of LPS. Together, these findings suggest a prominent role for GSH and its intracellular repartition in LPS anorexia.
Faculty
Faculté des sciences et de médecine
Department
Département de Médecine
Language
  • English
Classification
Biology
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/300578
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