Role for glutathione in the hyposensitivity of LPS-pretreated mice to LPS anorexia

Hernadfalvi, Noemi; Langhans, Wolfgang; Meyenburg, Claudia von; Onteniente, Brigitte; Richard, Denis; Arsenijevic, Denis

doi:10.1684/ecn.2007.0090

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Journal article

Role for glutathione in the hyposensitivity of LPS-pretreated mice to LPS anorexia

Hernadfalvi, Noemi Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland
Langhans, Wolfgang Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland
Meyenburg, Claudia von Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland
Onteniente, Brigitte INSERM UMR421, Creteil, France
Richard, Denis Department of Anatomy and Physiology, Laval University, Quebec, Canada
Arsenijevic, Denis Institute of Animal Sciences, ETH Zurich, Schwerzenbach, Switzerland - Division of Physiology, Department of Medicine, University of Fribourg, Switzerland

2007

Published in:

European Cytokine Network. - 2007, vol. 18, no. 2, p. 39-45

English To study the role of the redox state regulator glutathione (GSH) in bacterial lipopolysaccharide (LPS)-induced anorexia we measured GSH in liver, serum and brain in response to intraperitoneal (ip) lipopolysaccharide (LPS, 4μg/mouse) injection in LPS-naïve and LPS-pretreated (4 μg/mouse) mice. LPS reduced food intake in LPS-naïve mice and LPS pretreatment attenuated this effect. LPS reduced total reduced GSH at 24 hours after injection in LPS-naïve mice. On the other hand, LPS pretreatment caused an upregulation of GSH levels in brain and liver, and this was associated with a significant attenuation of LPS-induced anorexia. In addition, LPS pretreatment increased mitochondrial GSH levels in brain and liver. Pharmacological GSH depletion with diethylmaleate and L-buthionine sulfoximine in LPS-pretreated mice ablated the hyposensitivity to the anorexic effect of LPS. Together, these findings suggest a prominent role for GSH and its intracellular repartition in LPS anorexia.

Faculty

Faculté des sciences et de médecine

Department

Département de Médecine

Language