Journal article

(Strept)avidin as host for biotinylated coordination complexes: stability, chiral discrimination, and cooperativity

  • Loosli, Andreas Institute of Chemistry, University of Neuchâtel, Switzerland
  • Rusbandi, Untung Edy Institute of Chemistry, University of Neuchâtel, Switzerland
  • Gradinaru, Julieta Institute of Chemistry, University of Neuchâtel, Switzerland
  • Bernauer, Klaus Institute of Chemistry, University of Neuchâtel, Switzerland
  • Schläpfer, Carl-Wilhelm Institute of Inorganic Chemistry, University of Fribourg, Switzerland
  • Meyer, Michel Laboratoire d'Ingénierie Moléculaire pour la Séparation et les Applications des Gaz, Université de Bourgogne, Faculté des Sciences, France
  • Mazurek, Sylwester Institute of Chemistry, University of Neuchâtel, Switzerland - Laboratory of Chemometrics, National Institute of Chemistry, Ljubljana, Slovenia
  • Novic, Marjana Laboratory of Chemometrics, National Institute of Chemistry, Ljubljana, Slovenia
  • Ward, Thomas R. Institute of Chemistry, University of Neuchâtel, Switzerland
Show more…
    24.12.2005
Published in:
  • Inorganic Chemistry. - 2006, vol. 45, no. 2, p. 660-668
English Incorporation of a biotinylated ruthenium tris(bipyridine) [Ru(bpy)₂(Biot-bpy)]²⁺ (1) in either avidin or streptavidin-(strept)avidin-can be conveniently followed by circular dichroism spectroscopy. To determine the stepwise association constants, cooperativity, and chiral discrimination properties, diastereopure (Λ and δ)-1 species were synthesized and incorporated in tetrameric (strept)avidin to afford (δ-[Ru(bpy)₂(Biot-bpy)]²⁺)x⊂avidin, (Λ- [Ru(bpy)₂(Biot-bpy)]²⁺)x⊂avidin, (δ-[Ru(bpy)₂(Biot- bpy)]²⁺)x⊂streptavidin, and (Λ-[Ru(bpy)₂(Biot-bpy)]²⁺) x⊂streptavidin (x = 1-4) For these four systems, the overall stability constants are log β₄ = 28.6, 30.3, 36.2, and 36.4, respectively. Critical analysis of the CD titrations data suggests a strong cooperativity between the first and the second binding event (x = 1, 2) and a pronounced difference in affinity between avidin and streptavidin for the dicationic guest 1 as well as modest enantiodiscrimination properties with avidin as host.
Faculty
Faculté des sciences et de médecine
Department
Département de Chimie
Language
  • English
Classification
Chemistry
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/300492
Statistics

Document views: 8 File downloads:
  • schlaepfer_shb.pdf: 1