Journal article

The Mi-2 nucleosome-remodeling protein LET-418 is targeted via LIN-1/ETS to the promoter of lin-39/Hox during vulval development in C. elegans

  • Guerry, Frédéric Département de Biologie, Université de Fribourg, Switzerland
  • Marti, Claude-Olivier Département de Biologie, Université de Fribourg, Switzerland
  • Zhang, Yue Département de Biologie, Université de Fribourg, Switzerland
  • Moroni, Paolo S. Département de Biologie, Université de Fribourg, Switzerland
  • Jaquiéry, Emilie Département de Biologie, Université de Fribourg, Switzerland
  • Müller, Fritz Département de Biologie, Université de Fribourg, Switzerland
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    24.03.2007
Published in:
  • Developmental Biology. - 2007, vol. 206, no. 2, p. 469-479
English The fate of the vulval cells in Caenorhabditis elegans is specified, at least in part, through a highly conserved RTK/Ras mediated signaling cascade that negatively regulates the activity of the ETS-like transcription factor LIN-1. The Hox gene lin-39 functions downstream of both, the LIN-3/RTK/Ras pathway and LIN-1 and plays a pivotal role in controlling vulva cell competence and induction. Here we show that LET-418, a C. elegans ortholog of the human NuRD component Mi-2, negatively modulates the activity of lin-39. LET-418 interacts in vivo with specific regions in the promoter of lin-39 and this interaction depends on LIN-1. Our data provide evidence for a model in which LIN-1 recruits LET-418/Mi-2 as co-repressor to the promoter of lin-39, thereby restricting its activity to the basal levels required in the vulva precursor cells (VPCs) for normal vulval development. Thus, our data suggest that the interaction between LIN-1 and LET-418/Mi-2 may link RTK/Ras signaling with chromatin remodeling and gene expression.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/300449
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