Journal article

Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei

  • Bouzaidi-Tiali, Nabile Department of Biology/Cell and Developmental Biology, University of Fribourg, Switzerland
  • Aeby, Eric Department of Biology/Cell and Developmental Biology, University of Fribourg, Switzerland
  • Charrière, Fabien Department of Biology/Cell and Developmental Biology, University of Fribourg, Switzerland
  • Pusnik, Mascha Department of Biology/Cell and Developmental Biology, University of Fribourg, Switzerland
  • Schneider, André Department of Biology/Cell and Developmental Biology, University of Fribourg, Switzerland
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    13.09.2007
Published in:
  • The EMBO Journal. - 2007, vol. 26, no. 20, p. 4302–4312
English Mitochondrial tRNA import is widespread in eukaryotes. Yet, the mechanism that determines its specificity is unknown. Previous in vivo experiments using the tRNAsMet, tRNAIle and tRNALys have suggested that the T-stem nucleotide pair 51:63 is the main localization determinant of tRNAs in Trypanosoma brucei. In the cytosol-specific initiator tRNAMet, this nucleotide pair is identical to the main antideterminant that prevents interaction with cytosolic elongation factor (eEF1a). Here we show that ablation of cytosolic eEF1a, but not of initiation factor 2, inhibits mitochondrial import of newly synthesized tRNAs well before translation or growth is affected. tRNASec is the only other cytosol-specific tRNA in T. brucei. It has its own elongation factor and does not bind eEF1a. However, a mutant of the tRNASec expected to bind to eEF1a is imported into mitochondria. This import requires eEF1a and aminoacylation of the tRNA. Thus, for a tRNA to be imported into the mitochondrion of T. brucei, it needs to bind eEF1a, and it is this interaction that mediates the import specificity.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/300399
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