Journal article

Transgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions

  • Sieber, Patrick Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
  • Schorderet, Martine Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
  • Ryser, Ulrich Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
  • Buchala, Antony J. Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
  • Kolattukudy, Pappachan Departments of Biochemistry and Medical Biochemistry and Neurobiotechnology Center, Ohio State University, Columbus, Ohio, USA
  • Métraux, Jean-Pierre Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
  • Nawrath, Christiane Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
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    2000
Published in:
  • Plant Cell. - 2000, vol. 12, no. 5, p. 721-737
English A major structural component of the cuticle of plants is cutin. Analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. Therefore, transgenic Arabidopsis plants were generated that express and secrete a cutinase from Fusarium solani f sp pisi. Arabidopsis plants expressing the cutinase in the extracellular space showed an altered ultrastructure of the cuticle and an enhanced permeability of the cuticle to solutes. In addition, pollen could germinate on fully differentiated leaves of cutinase- expressing plants but not on control leaves. These differences coincided with strong postgenital organ fusions. The junctions of the fusions contained pectic polysaccharides. As fused organs grew apart from each other, organ deformations and protrusions of epidermal cells developed at positions with high mechanical stress. These results demonstrate that an intact cutin layer not only is important for plant– environment interactions but also prevents fusions between different plant organs and is therefore necessary for normal epidermal differentiation and organ formation.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/300124
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