Journal article

The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

  • Kurdyukov, Sergey Max-Planck-Institut für Züchtungsforschung, Köln, Germany - ARC Centre of Excellence for Integrative Legume Research, School of Environmental and Life Sciences, University of Newcastle, Callaghan, Australia
  • Faust, Andrea Max-Planck-Institut für Züchtungsforschung, Köln, Germany
  • Nawrath, Christiane Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland - Department of Plant Molecular Biology, University of Lausanne, Switzerland
  • Bär, Sascha Max-Planck-Institut für Züchtungsforschung, Köln, Germany
  • Voisin, Derry Max-Planck-Institut für Züchtungsforschung, Köln, Germany
  • Efremova, Nadia Max-Planck-Institut für Züchtungsforschung, Köln, Germany
  • Franke, Rochus Institut für Zelluläre and Molekulare Botanik, Universität Bonn, Germany
  • Schreiber, Lukas Institut für Zelluläre and Molekulare Botanik, Universität Bonn, Germany
  • Saedler, Heinz Max-Planck-Institut für Züchtungsforschung, Köln, Germany
  • Métraux, Jean-Pierre Department of Biology, Unit of Plant Biology, University of Fribourg, Switzerland
  • Yephremov, Alexander Max-Planck-Institut für Züchtungsforschung, Köln, Germany
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    13.01.2006
Published in:
  • The Plant Cell. - 2006, vol. 18, no. 321
English The outermost epidermal cell wall is specialized to withstand pathogens and natural stresses, and lipid-based cuticular polymers are the major barrier against incursions. The Arabidopsis thaliana mutant bodyguard (bdg), which exhibits defects characteristic of the loss of cuticle structure not attributable to a lack of typical cutin monomers, unexpectedly accumulates significantly more cell wall–bound lipids and epicuticular waxes than wild-type plants. Pleiotropic effects of the bdg mutation on growth, viability, and cell differentiation are also observed. BDG encodes a member of the α/ß-hydrolase fold protein superfamily and is expressed exclusively in epidermal cells. Using Strep-tag epitope-tagged BDG for mutant complementation and immunolocalization, we show that BDG is a polarly localized protein that accumulates in the outermost cell wall in the epidermis. With regard to the appearance and structure of the cuticle, the phenotype conferred by bdg is reminiscent of that of transgenic Arabidopsis plants that express an extracellular fungal cutinase, suggesting that bdg may be incapable of completing the polymerization of carboxylic esters in the cuticular layer of the cell wall or the cuticle proper. We propose that BDG codes for an extracellular synthase responsible for the formation of cuticle. The alternative hypothesis proposes that BDG controls the proliferation/differentiation status of the epidermis via an unknown mechanism.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biology
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/300091
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