Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi

Dwyer, Rex A.; Huitema, Edgar; Beyer, Katinka; Cvitanich, Cristina; Kelkar, Hemant; Ah Fong, Audrey M. V.; Gates, Krista; Roberts, Samuel; Yatzkan, Einat; Gaffney, Thomas; Law, Marcus; Testa, Antonino; Torto-Alalibo, Trudy; Zhang, Meng; Zheng, Li; Mueller, Elisabeth; Windass, John; Binder, Andres; Birch, Paul R. J.; Gisi, Ulrich; Govers, Francine; Gow, Neil A.; Mauch, Felix; West, Pieter van; Waugh, Mark E.; Yu, Jun; Boller, Thomas; Kamoun, Sophien; Lam, Stephen T.; Judelson, Howard S.

doi:10.1094/MPMI-18-0229

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Journal article

Large-scale gene discovery in the oomycete Phytophthora infestans reveals likely components of phytopathogenicity shared with true fungi

Dwyer, Rex A. Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Huitema, Edgar Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, U.S.A.
Beyer, Katinka Friedrich Miescher Institute, Basel, Switzerland
Cvitanich, Cristina Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, U.S.A.
Kelkar, Hemant Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Ah Fong, Audrey M. V. Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, U.S.A.
Gates, Krista Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Roberts, Samuel Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, U.S.A.
Yatzkan, Einat Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Gaffney, Thomas Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Law, Marcus Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Testa, Antonino Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, U.S.A.
Torto-Alalibo, Trudy Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, U.S.A.
Zhang, Meng Beijing Genomics Institute, Institute of Genetics, and Graduate School, Chinese Academy of Sciences, Beijing, China
Zheng, Li Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Mueller, Elisabeth Syngenta Limited, Jealott's Hill International Research Station, Bracknell, Berks, U.K.
Windass, John Syngenta Limited, Jealott's Hill International Research Station, Bracknell, Berks, U.K.
Binder, Andres Syngenta Crop Protection AG, Werk Stein, Schaffhauserstrasse, Stein, Switzerland
Birch, Paul R. J. Plant Pathogen Interactions Program, Scottish Crop Research Institute, Invergowrie, Dundee, Scotland
Gisi, Ulrich Syngenta Crop Protection AG, Werk Stein, Schaffhauserstrasse, Stein, Switzerland
Govers, Francine Laboratory of Phytopathology, Wageningen University, The Netherlands
Gow, Neil A. Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, U.K.
Mauch, Felix Department of Biology, University of Fribourg, Switzerland
West, Pieter van Department of Molecular and Cell Biology, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, U.K.
Waugh, Mark E. National Center for Genome Resources, Old Pecos Trail, Santa Fe, U.S.A
Yu, Jun Beijing Genomics Institute, Institute of Genetics, and Graduate School, Chinese Academy of Sciences, Beijing, China
Boller, Thomas Friedrich Miescher Institute, Basel, Switzerland
Kamoun, Sophien Department of Plant Pathology, The Ohio State University, Ohio Agricultural Research and Development Center, Wooster, U.S.A.
Lam, Stephen T. Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, U.S.A.
Judelson, Howard S. Department of Plant Pathology and Center for Plant Cell Biology, University of California, Riverside, U.S.A.

2005

Published in:

Molecular Plant-Microbe Interactions. - 2005, vol. 18(3), p. 229

English To overview the gene content of the important pathogen Phytophthora infestans, large-scale cDNA and genomic sequencing was performed. A set of 75,757 high-quality expressed sequence tags (ESTs) from P. infestans was obtained from 20 cDNA libraries representing a broad range of growth conditions, stress responses, and developmental stages. These included libraries from P. infestans–potato and –tomato interactions, from which 963 pathogen ESTs were identified. To complement the ESTs, onefold coverage of the P. infestans genome was obtained and regions of coding potential identified. A unigene set of 18,256 sequences was derived from the EST and genomic data and characterized for potential functions, stage-specific patterns of expression, and codon bias. Cluster analysis of ESTs revealed major differences between the expressed gene content of mycelial and spore-related stages, and affinities between some growth conditions. Comparisons with databases of fungal pathogenicity genes revealed conserved elements of pathogenicity, such as class III pectate lyases, despite the considerable evolutionary distance between oomycetes and fungi. Thirty-seven genes encoding components of flagella also were identified. Several genes not anticipated to occur in oomycetes were detected, including chitin synthases, phosphagen kinases, and a bacterial-type FtsZ cell-division protein. The sequence data described are available in a searchable public database.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language