The period length of fibroblast circadian gene expression varies widely among human individuals
- Brown, Steven A. Department of Molecular Biology, University of Geneva, Switzerland
- Fleury-Olela, Fabienne Department of Molecular Biology, University of Geneva, Switzerland
- Nagoshi, Emi Department of Biology—Howard Hughes Medical Institute, Brandeis University, Waltham, MA, USA
- Hauser, Conrad Division of Immunology and Allergy, University Hospital Geneva, Switzerland
- Juge, Cristiana Endocrine Unit, Laboratory of Molecular Endocrinology, University Hospital Geneva, Switzerland
- Meier, Christophe A. Endocrine Unit, Laboratory of Molecular Endocrinology, University Hospital Geneva, Switzerland
- Chicheportiche, Rachel Division of Immunology and Allergy, University Hospital Geneva, Switzerland
- Dayer, Jean-Michel Division of Immunology and Allergy, University Hospital Geneva, Switzerland
- Albrecht, Urs Department of Medicine, Section of Biochemistry, University of Fribourg, Switzerland
- Schibler, Ueli Department of Molecular Biology, University of Geneva, Switzerland
-
27.09.2005
Published in:
- PLoS Biology. - 2005, vol. 3, no. 10, p. e338
English
Mammalian circadian behavior is governed by a central clock in the suprachiasmatic nucleus of the brain hypothalamus, and its intrinsic period length is believed to affect the phase of daily activities. Measurement of this period length, normally accomplished by prolonged subject observation, is difficult and costly in humans. Because a circadian clock similar to that of the suprachiasmatic nucleus is present in most cell types, we were able to engineer a lentiviral circadian reporter that permits characterization of circadian rhythms in single skin biopsies. Using it, we have determined the period lengths of 19 human individuals. The average value from all subjects, 24.5 h, closely matches average values for human circadian physiology obtained in studies in which circadian period was assessed in the absence of the confounding effects of light input and sleep–wake cycle feedback. Nevertheless, the distribution of period lengths measured from biopsies from different individuals was wider than those reported for circadian physiology. A similar trend was observed when comparing wheel-running behavior with fibroblast period length in mouse strains containing circadian gene disruptions. In mice, inter-individual differences in fibroblast period length correlated with the period of running-wheel activity; in humans, fibroblasts from different individuals showed widely variant circadian periods. Given its robustness, the presented procedure should permit quantitative trait mapping of human period length.
- Faculty
- Faculté des sciences et de médecine
- Department
- Département de Biologie
- Language
-
- English
- Classification
- Biological sciences
- License
- License undefined
- Identifiers
-
- RERO DOC 5433
- DOI 10.1371/journal.pbio.0030338
- Persistent URL
- https://folia.unifr.ch/unifr/documents/299858
Statistics
Document views: 46
File downloads:
- 1_albrecht_plf.pdf: 153