Dissecting the ß-aminobutyric acid–induced priming phenomenon in Arabidopsis

Ton, Jurriaan; Jakab, Gabor; Toquin, Valérie; Flors, Victor; Iavicoli, Annalisa; Maeder, Muriel N.; Métraux, Jean-Pierre; Mauch-Mani, Brigitte

doi:10.1105/tpc.104.029728

Back

Journal article

Dissecting the ß-aminobutyric acid–induced priming phenomenon in Arabidopsis

Ton, Jurriaan Laboratory of Biochemistry, Department of Science, University of Neuchâtel, Switzerland - Section of Phytopathology, Faculty of Biology, Utrecht University, The Netherlands
Jakab, Gabor Laboratory of Biochemistry, Department of Science, University of Neuchâtel, Switzerland - Plant Biology Department of Biology, University of Fribourg, Switzerland
Toquin, Valérie Plant Biology Department of Biology, University of Fribourg, Switzerland
Flors, Victor Laboratory of Biochemistry, Department of Science, University of Neuchâtel, Switzerland
Iavicoli, Annalisa Plant Biology Department of Biology, University of Fribourg, Switzerland
Maeder, Muriel N. Plant Biology Department of Biology, University of Fribourg, Switzerland
Métraux, Jean-Pierre Plant Biology Department of Biology, University of Fribourg, Switzerland
Mauch-Mani, Brigitte Laboratory of Biochemistry, Department of Science, University of Neuchâtel, Switzerland

18.02.2005

Published in:

The Plant Cell. - 2005, vol. 17, no. 3, p. 987-999

English Plants treated with the nonprotein amino acid ß-aminobutyric acid (BABA) develop an enhanced capacity to resist biotic and abiotic stresses. This BABA-induced resistance (BABA-IR) is associated with an augmented capacity to express basal defense responses, a phenomenon known as priming. Based on the observation that high amounts of BABA induce sterility in Arabidopsis thaliana, a mutagenesis screen was performed to select mutants impaired in BABA-induced sterility (ibs). Here, we report the isolation and subsequent characterization of three T-DNA–tagged ibs mutants. Mutant ibs1 is affected in a cyclin-dependent kinase–like protein, and ibs2 is defective in AtSAC1b encoding a polyphosphoinositide phosphatase. Mutant ibs3 is affected in the regulation of the ABA1 gene encoding the abscisic acid (ABA) biosynthetic enzyme zeaxanthin epoxidase. To elucidate the function of the three IBS genes in plant resistance, the mutants were tested for BABA-IR against the bacterium Pseudomonas syringae pv tomato, the oomycete Hyaloperonospora parasitica, and BABA-induced tolerance to salt. All three ibs mutants were compromised in BABA-IR against H. parasitica, although to a different extent. Whereas ibs1 was reduced in priming for salicylate (SA)-dependent trailing necrosis, mutants ibs2 and ibs3 were affected in the priming for callose deposition. Only ibs1 failed to express BABA-IR against P. syringae, which coincided with a defect in priming for SA-inducible PR-1 gene expression. By contrast, ibs2 and ibs3 showed reduced BABA-induced tolerance to salt, which correlated with an affected priming for ABA-inducible gene expression. For all three ibs alleles, the defects in BABA-induced sterility and BABA-induced protection against P. syringae, H. parasitica, and salt could be confirmed in independent mutants. The data presented here introduce three novel regulatory genes involved in priming for different defense responses.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language