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Journal article

Gene-specific Involvement of ß-oxidation in wound-activated responses in Arabidopsis

  • Castillo, M. Cruz Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
  • Martínez, Cristina Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
  • Buchala, Antony J. Plant Biology, Department of Biology, University of Fribourg, Switzerland
  • Métraux, Jean-Pierre Plant Biology, Department of Biology, University of Fribourg, Switzerland
  • León, José Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain
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    01.05.2004
Published in:
  • Plant Physiology. - 2004, vol. 135, no. 1, p. 85-94
English The coordinated induced expression of ß-oxidation genes is essential to provide the energy supply for germination and postgerminative development. However, very little is known about other functions of ß-oxidation in nonreserve organs. We have identified a gene-specific pattern of induced ß-oxidation gene expression in wounded leaves of Arabidopsis. Mechanical damage triggered the local and systemic induction of only ACX1 among acyl-coenzyme A oxidase (ACX) genes, and KAT2/PED1 among 3-ketoacyl-coenzyme A thiolase (KAT) genes in Arabidopsis. In turn, wounding induced KAT5/PKT2 only systemically. Although most of the ß-oxidation genes were activated by wound-related factors such as dehydration and abscisic acid, jasmonic acid (JA) induced only ACX1 and KAT5. Reduced expression of ACX1 or KAT2 genes, in transgenic plants expressing their corresponding mRNAs in antisense orientation, correlated with defective wound-activated synthesis of JA and with reduced expression of JA-responsive genes. Induced expression of JA-responsive genes by exogenous application of JA was unaffected in those transgenic plants, suggesting that ACX1 and KAT2 play a major role in driving wound-activated responses by participating in the biosynthesis of JA in wounded Arabidopsis plants.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
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Persistent URL
https://folia.unifr.ch/unifr/documents/299691
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