Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism

Glombitza, Sabine; Dubuis, Pierre-Henri; Thulke, Oliver; Welzl, Gerhard; Bovet, Lucien; Götz, Michael; Affenzeller, Matthias; Geist, Birgit; Hehn, Alain; Asnaghi, Carole; Ernst, Dieter; Seidlitz, Harald; Gundlach, Heidrun; Mayer, Klaus; Martinoia, Enrico; Werck-reichhart, Daniele; Mauch, Felix; Schäffner, Anton

doi:10.1007/s11103-004-0274-3

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Journal article

Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism

Glombitza, Sabine Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Dubuis, Pierre-Henri Plant Biology, Department of Biology, University of Fribourg, Switzerland
Thulke, Oliver Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Welzl, Gerhard Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Bovet, Lucien Plant Biology, Department of Biology, University of Fribourg, Switzerland
Götz, Michael Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Affenzeller, Matthias Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Geist, Birgit Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Hehn, Alain Department of Plant Stress Response, Institute of Plant Molecular Biology, Université Louis Pasteur, Strasbourg, France
Asnaghi, Carole Plant Biology – Molecular Plant Physiology, University of Zurich, Switzerland
Ernst, Dieter Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Seidlitz, Harald Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Gundlach, Heidrun Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Mayer, Klaus Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
Martinoia, Enrico Plant Biology – Molecular Plant Physiology, University of Zurich, Switzerland
Werck-reichhart, Daniele Department of Plant Stress Response, Institute of Plant Molecular Biology, Université Louis Pasteur, F-67083 Strasbourg Cedex, France
Mauch, Felix Plant Biology, Department of Biology, University of Fribourg, Switzerland
Schäffner, Anton Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany

2004

Published in:

Plant Molecular Biology. - 2005, vol. 54, no. 6, p. 817-835

English Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively in secondary metabolism and defense reactions, a DNA array (MetArray) harboring gene-specific probes was established. It comprised complete sets of genes encoding 109 secondary product glycosyltransferases and 63 glutathione-utilizing enzymes along with 62 cytochrome P450 monooxygenases and 26 ABC transporters. Their transcriptome was monitored in different organs of unstressed plants and in shoots in response to herbicides, UV-B radiation, endogenous stress hormones, and pathogen infection. A principal component analysis based on the transcription of these effector gene families defined distinct responses and crosstalk. Methyl jasmonate and ethylene treatments were separated from a group combining reactions towards two sulfonylurea herbicides, salicylate and an avirulent strain of Pseudomonas syringae pv. tomato. The responses to the herbicide bromoxynil and UV-B radiation were distinct from both groups. In addition, these analyses pinpointed individual effector genes indicating their role in these stress responses. A small group of genes was diagnostic in differentiating the response to two herbicide classes used. Interestingly, a subset of genes induced by P. syringae was not responsive to the applied stress hormones. Small groups of comprehensively induced effector genes indicate common defense strategies. Furthermore, homologous members within branches of these effector gene families displayed differential expression patterns either in both organs or during stress responses arguing for their non-redundant functions.

Faculty

Faculté des sciences et de médecine

Department

Département de Biologie

Language