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Crosstalk and differential response to abiotic and biotic stressors reflected at the transcriptional level of effector genes from secondary metabolism

  • Glombitza, Sabine Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Dubuis, Pierre-Henri Plant Biology, Department of Biology, University of Fribourg, Switzerland
  • Thulke, Oliver Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Welzl, Gerhard Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Bovet, Lucien Plant Biology, Department of Biology, University of Fribourg, Switzerland
  • Götz, Michael Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Affenzeller, Matthias Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Geist, Birgit Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Hehn, Alain Department of Plant Stress Response, Institute of Plant Molecular Biology, Université Louis Pasteur, Strasbourg, France
  • Asnaghi, Carole Plant Biology – Molecular Plant Physiology, University of Zurich, Switzerland
  • Ernst, Dieter Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Seidlitz, Harald Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Gundlach, Heidrun Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Mayer, Klaus Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
  • Martinoia, Enrico Plant Biology – Molecular Plant Physiology, University of Zurich, Switzerland
  • Werck-reichhart, Daniele Department of Plant Stress Response, Institute of Plant Molecular Biology, Université Louis Pasteur, F-67083 Strasbourg Cedex, France
  • Mauch, Felix Plant Biology, Department of Biology, University of Fribourg, Switzerland
  • Schäffner, Anton Department of Environmental Engineering, Institute of Biochemical Plant Pathology, Institute of Developmental Genetics, Institute of Bioinformatics, MIPS, and Institute of Soil Ecology GSF – National Research Center for Environment and Health, Neuherberg, Germany
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    2004
Published in:
  • Plant Molecular Biology. - 2005, vol. 54, no. 6, p. 817-835
gene families
glutathione
dependent enzymes
glycosyltransferases
herbicides
pathogen
stress hormones
English Plant secondary metabolism significantly contributes to defensive measures against adverse abiotic and biotic cues. To investigate stress-induced, transcriptional alterations of underlying effector gene families, which encode enzymes acting consecutively in secondary metabolism and defense reactions, a DNA array (MetArray) harboring gene-specific probes was established. It comprised complete sets of genes encoding 109 secondary product glycosyltransferases and 63 glutathione-utilizing enzymes along with 62 cytochrome P450 monooxygenases and 26 ABC transporters. Their transcriptome was monitored in different organs of unstressed plants and in shoots in response to herbicides, UV-B radiation, endogenous stress hormones, and pathogen infection. A principal component analysis based on the transcription of these effector gene families defined distinct responses and crosstalk. Methyl jasmonate and ethylene treatments were separated from a group combining reactions towards two sulfonylurea herbicides, salicylate and an avirulent strain of Pseudomonas syringae pv. tomato. The responses to the herbicide bromoxynil and UV-B radiation were distinct from both groups. In addition, these analyses pinpointed individual effector genes indicating their role in these stress responses. A small group of genes was diagnostic in differentiating the response to two herbicide classes used. Interestingly, a subset of genes induced by P. syringae was not responsive to the applied stress hormones. Small groups of comprehensively induced effector genes indicate common defense strategies. Furthermore, homologous members within branches of these effector gene families displayed differential expression patterns either in both organs or during stress responses arguing for their non-redundant functions.
Faculty
Faculté des sciences et de médecine
Department
Département de Biologie
Language
  • English
Classification
Biological sciences
License
License undefined
Identifiers
Persistent URL
https://folia.unifr.ch/unifr/documents/299662
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